Automated Non-invasive Video-Microscopy of Oyster Spat Heart Rate during Acute Temperature Change: Impact of Acclimation Temperature.
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ABSTRACT:
SUBMITTER: Domnik NJ
PROVIDER: S-EPMC4916204 | biostudies-other | 2016
REPOSITORIES: biostudies-other
Publications
Automated Non-invasive Video-Microscopy of Oyster Spat Heart Rate during Acute Temperature Change: Impact of Acclimation Temperature.
Domnik Nicolle J NJ Polymeropoulos Elias T ET Elliott Nicholas G NG Frappell Peter B PB Fisher John T JT
Frontiers in physiology 20160622
We developed an automated, non-invasive method to detect real-time cardiac contraction in post-larval (1.1-1.7 mm length), juvenile oysters (i.e., oyster spat) via a fiber-optic trans-illumination system. The system is housed within a temperature-controlled chamber and video microscopy imaging of the heart was coupled with video edge-detection to measure cardiac contraction, inter-beat interval, and heart rate (HR). We used the method to address the hypothesis that cool acclimation (10°C vs. 22° ...[more]
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