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Cloned and expressed macrophage nitric oxide synthase contrasts with the brain enzyme.

ABSTRACT: Nitric oxide (NO) is a messenger molecule of macrophages, endothelial cells in blood vessels, and neurons. A neuronal form of NO synthase (NOS) has been previously cloned. We now report the molecular cloning of macrophage NOS. The macrophage enzyme displays 50% sequence identity to the neuronal enzyme. Like neuronal NOS, macrophage NOS has recognition sites for FAD, FMN, and NADPH and also has a consensus calmodulin binding site. Macrophage NOS mRNA is strikingly inducible; it is absent in quiescent macrophages or spleen but is prominent 2-6 hr after endotoxin treatment.

SUBMITTER: Lowenstein CJ

PROVIDER: S-EPMC49573 | biostudies-other | 1992 Aug

REPOSITORIES: biostudies-other

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Cloned and expressed macrophage nitric oxide synthase contrasts with the brain enzyme.

Lowenstein C J CJ Glatt C S CS Bredt D S DS Snyder S H SH

Proceedings of the National Academy of Sciences of the United States of America 19920801 15

Nitric oxide (NO) is a messenger molecule of macrophages, endothelial cells in blood vessels, and neurons. A neuronal form of NO synthase (NOS) has been previously cloned. We now report the molecular cloning of macrophage NOS. The macrophage enzyme displays 50% sequence identity to the neuronal enzyme. Like neuronal NOS, macrophage NOS has recognition sites for FAD, FMN, and NADPH and also has a consensus calmodulin binding site. Macrophage NOS mRNA is strikingly inducible; it is absent in quies ...[more]

PMID: 1379716

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