A simple, rapid, low-cost technique for naked-eye detection of urine-isolated TMPRSS2:ERG gene fusion RNA.
Ontology highlight
ABSTRACT:
SUBMITTER: Koo KM
PROVIDER: S-EPMC4965811 | biostudies-other | 2016
REPOSITORIES: biostudies-other
Publications
A simple, rapid, low-cost technique for naked-eye detection of urine-isolated TMPRSS2:ERG gene fusion RNA.
Koo Kevin M KM Wee Eugene J H EJ Mainwaring Paul N PN Trau Matt M
Scientific reports 20160729
The TMPRSS2:ERG gene fusion is one of a series of highly promising prostate cancer (PCa) biomarker alternatives to the controversial serum PSA. Current methods for detecting TMPRSS2:ERG are limited in terms of long processing time, high cost and the need for specialized equipment. Thus, there is an unmet need for less complex, faster, and cheaper methods to enable gene fusion detection in the clinic. We describe herein a simple, rapid and inexpensive assay which combines robust isothermal amplif ...[more]
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