Ontology highlight
ABSTRACT:
SUBMITTER: Whiten DR
PROVIDER: S-EPMC4981889 | biostudies-other | 2016
REPOSITORIES: biostudies-other
Whiten D R DR San Gil R R McAlary L L Yerbury J J JJ Ecroyd H H Wilson M R MR
Scientific reports 20160812
Proteinaceous cytoplasmic inclusions are an indicator of dysfunction in normal cellular proteostasis and a hallmark of many neurodegenerative diseases. We describe a simple and rapid new flow cytometry-based method to enumerate, characterise and, if desired, physically recover protein inclusions from cells. This technique can analyse and resolve a broad variety of inclusions differing in both size and protein composition, making it applicable to essentially any model of intracellular protein agg ...[more]