Ontology highlight
ABSTRACT:
SUBMITTER: Veeneman BA
PROVIDER: S-EPMC5006238 | biostudies-other | 2016 Jan
REPOSITORIES: biostudies-other
Veeneman Brendan A BA Shukla Sudhanshu S Dhanasekaran Saravana M SM Chinnaiyan Arul M AM Nesvizhskii Alexey I AI
Bioinformatics (Oxford, England) 20151030 1
<h4>Motivation</h4>Discovery of novel splicing from RNA sequence data remains a critical and exciting focus of transcriptomics, but reduced alignment power impedes expression quantification of novel splice junctions.<h4>Results</h4>Here, we profile performance characteristics of two-pass alignment, which separates splice junction discovery from quantification. Per sample, across a variety of transcriptome sequencing datasets, two-pass alignment improved quantification of at least 94% of simulate ...[more]