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In Vitro Evaluation of the Antioxidant Activity and Wound Healing Properties of Jaboticaba (Plinia peruviana) Fruit Peel Hydroalcoholic Extract.

ABSTRACT: Jaboticaba is a fruit from a native tree to Brazil, Plinia peruviana. Jaboticaba peels are an important source of antioxidant molecules such as phenolic compounds. This study aimed to evaluate in vitro the activity of a hydroalcoholic extract of jaboticaba fruit peels (HEJFP) in wound healing processes and antioxidant activity in murine fibroblasts (L929 cell line). HEJFP concentrations (0.5, 1, 5, 10, 25, 50, 100, and 200?µg/mL) were tested in MTT assay and cell proliferation was verified at 100?µg/mL after 24?h and at 25, 50, and 100?µg/mL after 48?h of extract exposure. Evaluation of antioxidant activity was performed at 0.5, 5, 25, 50, and 100?µg/mL HEJFP concentrations. Cell treatment with HEJFP at 25, 50, and 100?µg/mL for 24?h followed by H2O2 exposure for 3?h showed a strong cytoprotective effect. In vitro scratch wound healing assay indicated that none of tested HEJFP concentrations (0.5, 5, 25, 50, and 100?µg/mL) were capable of increasing migration rate after 12?h of incubation. These results demonstrate a positive effect of HEJFP on the wound healing process on L929 fibroblasts cell line, probably due to the antioxidant activity exhibited by phytochemicals in the extract.

SUBMITTER: Pitz Hda S

PROVIDER: S-EPMC5007430 | biostudies-other | 2016

REPOSITORIES: biostudies-other

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In Vitro Evaluation of the Antioxidant Activity and Wound Healing Properties of Jaboticaba (Plinia peruviana) Fruit Peel Hydroalcoholic Extract.

Pitz Heloisa da S Hda S Pereira Aline A Blasius Mayara B MB Voytena Ana Paula L AP Affonso Regina C L RC Fanan Simone S Trevisan Adriana C D AC Ribeiro-do-Valle Rosa M RM Maraschin Marcelo M

Oxidative medicine and cellular longevity 20160818

Jaboticaba is a fruit from a native tree to Brazil, Plinia peruviana. Jaboticaba peels are an important source of antioxidant molecules such as phenolic compounds. This study aimed to evaluate in vitro the activity of a hydroalcoholic extract of jaboticaba fruit peels (HEJFP) in wound healing processes and antioxidant activity in murine fibroblasts (L929 cell line). HEJFP concentrations (0.5, 1, 5, 10, 25, 50, 100, and 200 µg/mL) were tested in MTT assay and cell proliferation was verified at 10 ...[more]

PMID: 27630758

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Dataset Information

In Vitro Evaluation of the Antioxidant Activity and Wound Healing Properties of Jaboticaba (Plinia peruviana) Fruit Peel Hydroalcoholic Extract.

Publications

In Vitro Evaluation of the Antioxidant Activity and Wound Healing Properties of Jaboticaba (Plinia peruviana) Fruit Peel Hydroalcoholic Extract.

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