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Maesa indica: a nutritional wild berry rich in polyphenols with special attention to radical scavenging and inhibition of key enzymes, ?-amylase and ?-glucosidase.

ABSTRACT: The present study was aimed to evaluate the nutritional, antioxidant properties and inhibition of the key enzyme such as ?-amylase and ?-glucosidase from the fruits of Maesa indica. The results revealed that M. indica fruits possess an enormous amount of protein (45.68 mg/g), carbohydrates (25.12 mg/g) and mineral elements. The acetone extract were capable of hunting radicals by providing electrons and break chain reaction, especially in ABTS·+ (3719.23 µmol TE/g extract), OH· (66.50 %) and NO· (81.50 %) radical scavenging assays. The methanol extract showed a strong inhibition towards ?-amylase and ?-glucosidase (IC50 of 37.80 and 23.74 µg/mL, respectively). HPLC analysis enumerate that both extracts illustrates the presence of polyphenolic compounds namely quercetin, caffeic acid, rutin and chlorogenic acid.

SUBMITTER: Shanmugam S

PROVIDER: S-EPMC5052164 | biostudies-other | 2016 Jul

REPOSITORIES: biostudies-other

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Maesa indica: a nutritional wild berry rich in polyphenols with special attention to radical scavenging and inhibition of key enzymes, α-amylase and α-glucosidase.

Shanmugam Saravanan S Baby John Prakash JP Chandran Rahul R Thankarajan Sajeesh S Thangaraj Parimelazhagan P

Journal of food science and technology 20160723 7

The present study was aimed to evaluate the nutritional, antioxidant properties and inhibition of the key enzyme such as α-amylase and α-glucosidase from the fruits of Maesa indica. The results revealed that M. indica fruits possess an enormous amount of protein (45.68 mg/g), carbohydrates (25.12 mg/g) and mineral elements. The acetone extract were capable of hunting radicals by providing electrons and break chain reaction, especially in ABTS·+ (3719.23 µmo ...[more]

PMID: 27765966

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Project description:Diabetes mellitus is a chronic disease that is becoming a serious global health problem. Diabetes has been considered to be one of the major risks of cataract and retinopathy. Synthetic and natural product inhibitors of carbohydrate degrading enzymes are able to reduce type 2 diabetes and its complications. For a long time, potatoes have been portrayed as unhealthy for diabetic patients by some nutritionist due to their high starch content. However, purple and red potato cultivars have received considerable attention from consumers because they have high levels of polyphenolic compounds that have potent antioxidant activities. In this study, we screened the total phenolics (TP) and total anthocyanins (TA) and analyzed the phenolic and anthocyanin compounds in selected potato cultivars and advanced selections with distinct flesh colors (purple, red, yellow and white). Purple and red potato cultivars had higher levels of TP and TA than tubers with other flesh colors. Chlorogenic acid is the predominant phenolic acid, and major anthocyanin is composed of the derivatives of petunidin, peonidin, malvidin and pelargonidin. We tested the potential inhibitory effect of potato extracts on the activities of ?-amylase and ?-glucosidase, which were targeted to develop antidiabetic therapeutic agents. We also measured inhibitory effect of potato extracts on aldose reductase (AR) which is a key enzyme that has been a major drug target for the development of therapies to treat diabetic complications. Purple flesh tubers extract showed the most effective inhibition of ?-amylase, ?-glucosidase, and aldose reductase with IC50 values 25, 42, and 32 ?g/ml, respectively. Kinetic studies showed that anthocyanins are noncompetitive inhibitors of these enzymes, whereas phenolic acids behaved as mixed inhibitors for ?-amylase and ?-glucosidase and noncompetitive inhibitors for AR. This study supports the development of a positive and healthful image of potatoes, which is an important issue for consumers.

Dataset Information

Maesa indica: a nutritional wild berry rich in polyphenols with special attention to radical scavenging and inhibition of key enzymes, ?-amylase and ?-glucosidase.

Publications

<i>Maesa indica</i>: a nutritional wild berry rich in polyphenols with special attention to radical scavenging and inhibition of key enzymes, <i>α</i>-amylase and <i>α</i>-glucosidase.

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