Alphaherpesvirus DNA replication in dissociated human trigeminal ganglia.
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ABSTRACT:
SUBMITTER: Cohrs RJ
PROVIDER: S-EPMC5055419 | biostudies-other | 2016 Oct
REPOSITORIES: biostudies-other
Publications
Alphaherpesvirus DNA replication in dissociated human trigeminal ganglia.
Cohrs Randall J RJ Badani Hussain H Bos Nathan N Scianna Charles C Hoskins Ian I Baird Nicholas L NL Gilden Don D
Journal of neurovirology 20160512 5
Analysis of the frequency and PCR-quantifiable abundance of herpes simplex virus type 1 (HSV-1) and varicella zoster virus (VZV) DNA in multiple biological replicates of cells from dissociated randomly distributed human trigeminal ganglia (TG) of four subjects revealed an increase in both parameters and in both viruses during 5 days of culture, with no further change by 10 days. Dissociated TG provides a platform to analyze initiation of latent virus DNA replication within 5 days of culture. ...[more]
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