Dataset Information

A network approach to discerning the identities of C. elegans in a free moving population.

ABSTRACT: The study of C. elegans has led to ground-breaking discoveries in gene-function, neuronal circuits, and physiological responses. Subtle behavioral phenotypes, however, are often difficult to measure reproducibly. We have developed an experimental and computational infrastructure to simultaneously record and analyze the physical characteristics, movement, and social behaviors of dozens of interacting free-moving nematodes. Our algorithm implements a directed acyclic network that reconstructs the complex behavioral trajectories generated by individual C. elegans in a free moving population by chaining hundreds to thousands of short tracks into long contiguous trails. This technique allows for the high-throughput quantification of behavioral characteristics that require long-term observation of individual animals. The graphical interface we developed will enable researchers to uncover, in a reproducible manner, subtle time-dependent behavioral phenotypes that will allow dissection of the molecular mechanisms that give rise to organism-level behavior.

SUBMITTER: Winter PB

PROVIDER: S-EPMC5057085 | biostudies-other | 2016 Oct

REPOSITORIES: biostudies-other

ACCESS DATA

Publications

A network approach to discerning the identities of C. elegans in a free moving population.

Winter Peter B PB Brielmann Renee M RM Timkovich Nicholas P NP Navarro Helio T HT Teixeira-Castro Andreia A Morimoto Richard I RI Amaral Luis A N LA

Scientific reports 20161011

The study of C. elegans has led to ground-breaking discoveries in gene-function, neuronal circuits, and physiological responses. Subtle behavioral phenotypes, however, are often difficult to measure reproducibly. We have developed an experimental and computational infrastructure to simultaneously record and analyze the physical characteristics, movement, and social behaviors of dozens of interacting free-moving nematodes. Our algorithm implements a directed acyclic network that reconstructs the ...[more]

PMID: 27725712

Similar Datasets

Project description:How are evaluative reactions pertaining post-national citizenship identities interrelated and what are the potential mechanisms how post-national identities evolve? Previous efforts to operationalize and measure post-national citizenship identities leave it open how people's stances on different issues are related and suffer from a variety of theoretical and methodological shortcomings regarding the nature of political attitudes and ideologies. A recently proposed approach conceptualizes ideologies as networks of causally connected evaluative reactions to individual issues. Individual evaluative reactions form the nodes in a network model, and these nodes can influence each other via linked edges, thereby giving rise to a dynamic thoughts system of networked political and identity-related views. To examine this system at large, we apply network analysis to data from the European Values Study. Specifically, we investigate 33 evaluative reactions regarding national and supra-national identity, diversity, global empathy, global environmentalism, immigration, and supra-national politics. The results reveal a strongly connected network of citizenship identity-related attitudes. A community analysis reveals larger clusters of strongly related evaluative reactions, which are connected via bridges and hub nodes. Centrality analysis identifies evaluative reactions that are strategically positioned in the network, and network simulations indicate that persuasion attempts targeted at such nodes have greater potential to influence the larger citizenship identity than changes of more peripheral attitude nodes. We lastly show that socio-demographic characteristics are not only associated with the overall level of post-national citizenship, but also with the network structure, suggesting that these structural differences can affect the network function as people develop national or post-national citizenship identities, or respond to external events. These results provide new insights into the structure of post-national identities and the mechanism how post-national identities might evolve. We end with a discussion of future opportunities to study networked attitudes in the context of civic and citizenship education.

Dataset Information

A network approach to discerning the identities of C. elegans in a free moving population.

Publications

A network approach to discerning the identities of C. elegans in a free moving population.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets