Dataset Information

Primary structure and ontogeny of an avian CD3 transcript.

ABSTRACT: The structure of a chicken CD3 chain has been determined by isolating a cDNA clone (T11.15) that encodes a 175-amino-acid-long protein, including the NH2-terminal signal peptide. In Northern blot experiments, the earliest expression of the T11.15 transcript was detected in the thymus at embryonic day 10 (i.e., 1 day after cytoplasmic expression of a CD3 epitope recognized by a specific monoclonal antibody [CT3; Chen, C.L.H., Ager, L.L., Gartland, G.L. & Cooper, M.D. (1986) J. Exp. Med. 164, 375-380], but 2 days before the appearance of clonotypic components of the T-cell antigen receptor). Sequence similarity of this chicken protein sequence compared with that of the known mammalian CD3 gamma and delta polypeptides was 36-39% and 39-40%, respectively. Amino acid sequence alignments between avian and mammalian CD3 revealed maximum conservation in the transmembrane and cytoplasmic domains as well as in the regions flanking the cysteine residues in the extracellular domain, underlining their functional importance. The difficulty of unambiguously assigning this chain to a single mammalian CD3 subunit on the basis of sequence comparison raises the possibility that this polypeptide represents a derivative from an ancestral form of the gamma and delta chains. It is thus possible that a single chain may play the role of both CD3 gamma and delta subunits in the chicken CD3 complex or, alternatively, that gene duplications occurred independently in the avian and mammalian lineages.

SUBMITTER: Bernot A

PROVIDER: S-EPMC51270 | biostudies-other | 1991 Mar

REPOSITORIES: biostudies-other

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Primary structure and ontogeny of an avian CD3 transcript.

Bernot A A Auffray C C

Proceedings of the National Academy of Sciences of the United States of America 19910301 6

The structure of a chicken CD3 chain has been determined by isolating a cDNA clone (T11.15) that encodes a 175-amino-acid-long protein, including the NH2-terminal signal peptide. In Northern blot experiments, the earliest expression of the T11.15 transcript was detected in the thymus at embryonic day 10 (i.e., 1 day after cytoplasmic expression of a CD3 epitope recognized by a specific monoclonal antibody [CT3; Chen, C.L.H., Ager, L.L., Gartland, G.L. & Cooper, M.D. (1986) J. Exp. Med. 164, 375- ...[more]

PMID: 1826056

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Project description:UnlabelledIn order to understand factors that may influence latency-associated transcription and latency-associated transcript (LAT) phenotypes, we studied the expression of the herpes simplex virus 2 (HSV-2) LAT-associated microRNAs (miRNAs). We mapped the transcription initiation sites of all three primary miRNA transcripts and identified the ICP4-binding sequences at the transcription initiation sites of both HSV-2 LAT (pri-miRNA for miR-I and miR-II, which target ICP34.5, and miR-III, which targets ICP0) and L/ST (a pri-miRNA for miR-I and miR-II) but not at that of the primary miR-H6 (for which the target is unknown). We confirmed activity of the putative HSV-2 L/ST promoter and found that ICP4 trans-activates the L/ST promoter when the ICP4-binding site at its transcription initiation site is mutated, suggesting that ICP4 may play a dual role in regulating transcription of L/ST and, consequently, of miR-I and miR-II. LAT exon 1 (containing LAT enhancer sequences), together with the LAT promoter region, comprises a bidirectional promoter required for the expression of both LAT-encoded miRNAs and miR-H6 in latently infected mouse ganglia. The ability of ICP4 to suppress ICP34.5-targeting miRNAs and to activate lytic viral genes suggests that ICP4 could play a key role in the switch between latency and reactivation.ImportanceThe HSV-2 LAT and viral miRNAs expressed in the LAT region are the most abundant viral transcripts during HSV latency. The balance between the expression of LAT and LAT-associated miRNAs and the expression of lytic viral transcripts from the opposite strand appears to influence whether individual HSV-infected neurons will be latently or productively infected. The outcome of neuronal infection may thus depend on regulation of gene expression of the corresponding primary miRNAs. In the present study, we characterize promoter sequences responsible for miRNA expression, including identification of the primary miRNA 5' ends and evaluation of ICP4 response. These findings provide further insight into the virus' strategy to tightly control expression of lytic cycle genes (especially the neurovirulence factor, ICP34.5) and suggest a mechanism (via ICP4) for the transition from latency to reactivated productive infection.

Dataset Information

Primary structure and ontogeny of an avian CD3 transcript.

Publications

Primary structure and ontogeny of an avian CD3 transcript.

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