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Programmed necrosis - a new mechanism of steroidogenic luteal cell death and elimination during luteolysis in cows.


ABSTRACT: Programmed necrosis (necroptosis) is an alternative form of programmed cell death that is regulated by receptor-interacting protein kinase (RIPK) 1 and 3-dependent, but is a caspase (CASP)-independent pathway. In the present study, to determine if necroptosis participates in bovine structural luteolysis, we investigated RIPK1 and RIPK3 expression throughout the estrous cycle, during prostaglandin F2? (PGF)-induced luteolysis in the bovine corpus luteum (CL), and in cultured luteal steroidogenic cells (LSCs) after treatment with selected luteolytic factors. In addition, effects of a RIPK1 inhibitor (necrostatin-1, Nec-1; 50??M) on cell viability, progesterone secretion, apoptosis related factors and RIPKs expression, were evaluated. Expression of RIPK1 and RIPK3 increased in the CL tissue during both spontaneous and PGF-induced luteolysis (P?

SUBMITTER: Hojo T 

PROVIDER: S-EPMC5128806 | biostudies-other | 2016 Nov

REPOSITORIES: biostudies-other

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Programmed necrosis - a new mechanism of steroidogenic luteal cell death and elimination during luteolysis in cows.

Hojo Takuo T   Siemieniuch Marta J MJ   Lukasik Karolina K   Piotrowska-Tomala Katarzyna K KK   Jonczyk Agnieszka W AW   Okuda Kiyoshi K   Skarzynski Dariusz J DJ  

Scientific reports 20161130


Programmed necrosis (necroptosis) is an alternative form of programmed cell death that is regulated by receptor-interacting protein kinase (RIPK) 1 and 3-dependent, but is a caspase (CASP)-independent pathway. In the present study, to determine if necroptosis participates in bovine structural luteolysis, we investigated RIPK1 and RIPK3 expression throughout the estrous cycle, during prostaglandin F2α (PGF)-induced luteolysis in the bovine corpus luteum (CL), and in cultured luteal steroidogenic  ...[more]

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