Unknown

Dataset Information

0

Cytochrome P450 family 4 in a cockroach: molecular cloning and regulation by regulation by hypertrehalosemic hormone.


ABSTRACT: Hypertrehalosemic hormone (a carbohydrate-mobilizing neuroendocrine decapeptide) and starvation markedly increased levels of a cockroach (Blaberus discoidalis) fat body cytochrome P450 message. The gene represented by the cloned P450 cDNA has been named CYP4C1 (cytochrome P450 family 4, subfamily C, gene 1), a newly identified member of the ubiquitous cytochrome P450 monooxygenase gene superfamily. Blaberus CYP4C1 (511 amino acids, Mr = 58,485) has a hydrophobic NH2 terminus and a sequence near the COOH terminus that is homologous to the cysteine-containing heme-binding region definitive of cytochromes P450. The cockroach sequence is 32-36% identical to mammalian family 4A and 4B enzymes. It contains a 13-residue sequence characteristic of family 4 but not other P450s. This study suggests that CYP4C1 is hormonally regulated in association with energy substrate mobilization and supports the idea that family 4 is an old and widespread gene family.

SUBMITTER: Bradfield JY 

PROVIDER: S-EPMC51700 | biostudies-other | 1991 May

REPOSITORIES: biostudies-other

altmetric image

Publications

Cytochrome P450 family 4 in a cockroach: molecular cloning and regulation by regulation by hypertrehalosemic hormone.

Bradfield J Y JY   Lee Y H YH   Keeley L L LL  

Proceedings of the National Academy of Sciences of the United States of America 19910501 10


Hypertrehalosemic hormone (a carbohydrate-mobilizing neuroendocrine decapeptide) and starvation markedly increased levels of a cockroach (Blaberus discoidalis) fat body cytochrome P450 message. The gene represented by the cloned P450 cDNA has been named CYP4C1 (cytochrome P450 family 4, subfamily C, gene 1), a newly identified member of the ubiquitous cytochrome P450 monooxygenase gene superfamily. Blaberus CYP4C1 (511 amino acids, Mr = 58,485) has a hydrophobic NH2 terminus and a sequence near  ...[more]

Similar Datasets

| S-EPMC384689 | biostudies-literature
| S-EPMC8588036 | biostudies-literature
| PRJEB38781 | ENA
| S-EPMC4807407 | biostudies-literature
| S-EPMC45864 | biostudies-other
| S-EPMC5634052 | biostudies-literature
| S-EPMC2533730 | biostudies-literature
| S-EPMC3578826 | biostudies-literature
| S-EPMC29286 | biostudies-literature
| S-EPMC9260165 | biostudies-literature