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Split Spinach Aptamer for Highly Selective Recognition of DNA and RNA at Ambient Temperatures.

ABSTRACT: Split spinach aptamer (SSA) probes for fluorescent analysis of nucleic acids were designed and tested. In SSA design, two RNA or RNA/DNA strands hybridized to a specific nucleic acid analyte and formed a binding site for low-fluorescent 3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI) dye, which resulted in up to a 270-fold increase in fluorescence. The major advantage of the SSA over state-of-the art fluorescent probes is high selectivity: it produces only background fluorescence in the presence of a single-base-mismatched analyte, even at room temperature. SSA is therefore a promising tool for label-free analysis of nucleic acids at ambient temperatures.

SUBMITTER: Kikuchi N

PROVIDER: S-EPMC5198575 | biostudies-other | 2016 Sep

REPOSITORIES: biostudies-other

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Split Spinach Aptamer for Highly Selective Recognition of DNA and RNA at Ambient Temperatures.

Kikuchi Nanami N Kolpashchikov Dmitry M DM

Chembiochem : a European journal of chemical biology 20160715 17

Split spinach aptamer (SSA) probes for fluorescent analysis of nucleic acids were designed and tested. In SSA design, two RNA or RNA/DNA strands hybridized to a specific nucleic acid analyte and formed a binding site for low-fluorescent 3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI) dye, which resulted in up to a 270-fold increase in fluorescence. The major advantage of the SSA over state-of-the art fluorescent probes is high selectivity: it produces only background fluorescence in the ...[more]

PMID: 27305425

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