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Large-scale exploration of growth inhibition caused by overexpression of genomic fragments in Saccharomyces cerevisiae.

ABSTRACT: We have screened the genome of Saccharomyces cerevisiae for fragments that confer a growth-retardation phenotype when overexpressed in a multicopy plasmid with a tetracycline-regulatable (Tet-off) promoter. We selected 714 such fragments with a mean size of 700 base-pairs out of around 84,000 clones tested. These include 493 in-frame open reading frame fragments corresponding to 454 distinct genes (of which 91 are of unknown function), and 162 out-of-frame, antisense and intergenic genomic fragments, representing the largest collection of toxic inserts published so far in yeast.

SUBMITTER: Boyer J

PROVIDER: S-EPMC522879 | biostudies-other | 2004

REPOSITORIES: biostudies-other

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Large-scale exploration of growth inhibition caused by overexpression of genomic fragments in Saccharomyces cerevisiae.

Boyer Jeanne J Badis Gwenaël G Fairhead Cécile C Talla Emmanuel E Hantraye Florence F Fabre Emmanuelle E Fischer Gilles G Hennequin Christophe C Koszul Romain R Lafontaine Ingrid I Ozier-Kalogeropoulos Odile O Ricchetti Miria M Richard Guy-Franck GF Thierry Agnès A Dujon Bernard B

Genome biology 20040831 9

We have screened the genome of Saccharomyces cerevisiae for fragments that confer a growth-retardation phenotype when overexpressed in a multicopy plasmid with a tetracycline-regulatable (Tet-off) promoter. We selected 714 such fragments with a mean size of 700 base-pairs out of around 84,000 clones tested. These include 493 in-frame open reading frame fragments corresponding to 454 distinct genes (of which 91 are of unknown function), and 162 out-of-frame, antisense and intergenic genomic fragm ...[more]

PMID: 15345056

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