Ontology highlight
ABSTRACT:
SUBMITTER: Gibbons IR
PROVIDER: S-EPMC52549 | biostudies-other | 1991 Oct
REPOSITORIES: biostudies-other
Gibbons I R IR Asai D J DJ Ching N S NS Dolecki G J GJ Mocz G G Phillipson C A CA Ren H H Tang W J WJ Gibbons B H BH
Proceedings of the National Academy of Sciences of the United States of America 19911001 19
A procedure that uses the PCR to make rapid successive steps through a random-primed cDNA library has been developed to provide a method for sequencing very long genes that are difficult to obtain as a single clone. In each successive step, the portions of partial clones that extend out from the region of known DNA sequence are amplified by two stages of PCR with nested, outward-directed primers designed approximately 50 bases in from the end of the known sequence, together with a general primer ...[more]