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Characterization of cDNA encoding a human sperm membrane protein related to A4 amyloid protein.

ABSTRACT: A rat testis lambda gt11 cDNA library was screened with a monoclonal antibody raised against a human sperm membrane protein designated YWK-II. A clone was found with a cDNA insert composed of 1837 base pairs that contained an open reading frame coding for 191 amino acid residues. The deduced polypeptide contained a segment with high homology to the transmembrane-cytoplasmic domains of the A4 amyloid protein found in brain plaques of Alzheimer disease patients. A sequence of basic amino acid residues, Arg-Lys-Arg, was found instead of Lys-Lys-Lys at the probable membrane-cytoplasmic junction that may be a unique property of sperm membrane proteins.

SUBMITTER: Yan YC

PROVIDER: S-EPMC53697 | biostudies-other | 1990 Apr

REPOSITORIES: biostudies-other

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Characterization of cDNA encoding a human sperm membrane protein related to A4 amyloid protein.

Yan Y C YC Bai Y Y Wang L F LF Miao S Y SY Koide S S SS

Proceedings of the National Academy of Sciences of the United States of America 19900401 7

A rat testis lambda gt11 cDNA library was screened with a monoclonal antibody raised against a human sperm membrane protein designated YWK-II. A clone was found with a cDNA insert composed of 1837 base pairs that contained an open reading frame coding for 191 amino acid residues. The deduced polypeptide contained a segment with high homology to the transmembrane-cytoplasmic domains of the A4 amyloid protein found in brain plaques of Alzheimer disease patients. A sequence of basic amino acid resi ...[more]

PMID: 1690887

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