Ontology highlight
ABSTRACT:
SUBMITTER: Schifferer M
PROVIDER: S-EPMC5404835 | biostudies-other | 2017 Apr
REPOSITORIES: biostudies-other
Cell chemical biology 20170330 4
Despite the urgent need for assays to visualize insulin secretion there is to date no reliable method available for measuring insulin release from single cells. To address this need, we developed a genetically encoded reporter termed RINS1 based on proinsulin superfolder GFP (sfGFP) and mCherry fusions for monitoring insulin secretion. RINS1 expression in MIN6 β cells resulted in proper processing yielding single-labeled insulin species. Unexpectedly, glucose or drug stimulation of insulin secre ...[more]