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Comparison of the sevenless genes of Drosophila virilis and Drosophila melanogaster.

ABSTRACT: The sevenless gene of Drosophila melanogaster encodes a transmembrane tyrosine kinase receptor required for normal eye development. We report here the isolation and DNA sequence analysis of the sevenless gene from Drosophila virilis. The predicted amino acid sequences of the sevenless proteins from these two species, which diverged approximately 60 million years ago, are compared.

SUBMITTER: Michael WM

PROVIDER: S-EPMC54321 | biostudies-other | 1990 Jul

REPOSITORIES: biostudies-other

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Comparison of the sevenless genes of Drosophila virilis and Drosophila melanogaster.

Michael W M WM Bowtell D D DD Rubin G M GM

Proceedings of the National Academy of Sciences of the United States of America 19900701 14

The sevenless gene of Drosophila melanogaster encodes a transmembrane tyrosine kinase receptor required for normal eye development. We report here the isolation and DNA sequence analysis of the sevenless gene from Drosophila virilis. The predicted amino acid sequences of the sevenless proteins from these two species, which diverged approximately 60 million years ago, are compared. ...[more]

PMID: 2115169

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Structural and functional comparisons of the Drosophila virilis and Drosophila melanogaster rough genes.

Project description:We have isolated the homeobox gene rough (ro) from Drosophila virilis. Comparison of the predicted amino acid sequences of the D. melanogaster and D. virilis rough proteins reveals that domains of high conservation, including the homeodomain, are interspersed with highly diverged regions. Stretches of significant sequence conservation are also observed in the 5' promoter region and in the introns. The D. virilis rough gene rescues the rough mutant phenotype and is properly regulated when introduced into the D. melanogaster genome. Thus the rough protein as well as the cis-regulatory elements that ensure proper temporal and spatial regulation are functionally conserved between these Drosophila species.

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Evolution of gene position: chromosomal arrangement and sequence comparison of the Drosophila melanogaster and Drosophila virilis sina and Rh4 genes.

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Comparison of K+-channel genes within the genomes of Anopheles gambiae and Drosophila melanogaster.

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| S-EPMC24368 | biostudies-literature

The interaction of bride of sevenless with sevenless is conserved between Drosophila virilis and Drosophila melanogaster.

Project description:An inductive interaction between the sevenless (sev) transmembrane tyrosine kinase receptor and the bride of sevenless (boss) transmembrane ligand is required for the development of the R7 photoreceptor neuron in the compound eye of Drosophila melanogaster. The boss protein is proposed to contain a large N-terminal extracellular domain, seven transmembrane segments, and a C-terminal cytoplasmic tail. The boss protein from Drosophila virilis (bossvir) retains strong amino acid identity with loss from D. melanogaster (bossmel): 73% identity in the N-terminal extracellular domain and 91% identity in the seven-transmembrane domain, including the cytoplasmic tail. By using P-element-mediated DNA transformation, the bossmel and bossvir genes were shown to rescue the D. melanogaster boss1 mutation. The expression of bossvir protein in D. melanogaster is indistinguishable from that of bossmel protein. Noncoding sequences which may regulate boss expression were identified based on their conservation during evolution. The predicted sev protein from D. virilis (sevvir) was previously shown to be 63% identical to sev from D. melanogaster (sevmel). A chimeric gene, (sevvir/mel), encoding the extracellular domain of sevvir and the cytoplasmic domain of sevmel rescues the D. melanogaster sevd2 mutation through interaction with either bossvir or bossmel.

| S-EPMC46651 | biostudies-other

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