Nucleic acid detection using BRET-beacons based on bioluminescent protein-DNA hybrids.
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ABSTRACT:
SUBMITTER: Engelen W
PROVIDER: S-EPMC5436041 | biostudies-other | 2017 Mar
REPOSITORIES: biostudies-other
Publications
Nucleic acid detection using BRET-beacons based on bioluminescent protein-DNA hybrids.
Engelen Wouter W van de Wiel Kayleigh M KM Meijer Lenny H H LHH Saha Bedabrata B Merkx Maarten M
Chemical communications (Cambridge, England) 20170301 19
Bioluminescent molecular beacons have been developed using a modular design approach that relies on BRET between the bright luciferase NanoLuc and a Cy3 acceptor. While classical molecular beacons are hampered by background fluorescence and scattering, these BRET-beacons allow detection of low pM concentrations of nucleic acids directly in complex media. ...[more]
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