Unknown

Dataset Information

0

Molecular cloning and characterization of human pyruvate dehydrogenase beta subunit gene.


ABSTRACT: A genomic clone encompassing the entire gene for the human pyruvate dehydrogenase beta subunit (PDH beta) has been isolated by screening a leukocyte genomic library with a nick-translated human foreskin fibroblast PDH beta cDNA probe. The 18-kilobase clone was characterized by restriction enzyme analysis, extensive DNA sequencing, and primer-extension analysis. The PDH beta structural gene is composed of 10 exons and 9 introns. All intron-exon splice junctions follow the GT/AG rule. The Alu family was found in introns 2 and 8. The 5' flanking region of the PDH beta gene contains a "CAAT" consensus promoter sequence but no "TATA" sequence. Primer-extension analysis indicated that the PDH beta gene transcription start site is an adenine residue located 132 bases upstream from the initiation codon in exon 1.

SUBMITTER: Koike K 

PROVIDER: S-EPMC54373 | biostudies-other | 1990 Aug

REPOSITORIES: biostudies-other

altmetric image

Publications

Molecular cloning and characterization of human pyruvate dehydrogenase beta subunit gene.

Koike K K   Urata Y Y   Koike M M  

Proceedings of the National Academy of Sciences of the United States of America 19900801 15


A genomic clone encompassing the entire gene for the human pyruvate dehydrogenase beta subunit (PDH beta) has been isolated by screening a leukocyte genomic library with a nick-translated human foreskin fibroblast PDH beta cDNA probe. The 18-kilobase clone was characterized by restriction enzyme analysis, extensive DNA sequencing, and primer-extension analysis. The PDH beta structural gene is composed of 10 exons and 9 introns. All intron-exon splice junctions follow the GT/AG rule. The Alu fami  ...[more]

Similar Datasets

| S-EPMC1482817 | biostudies-literature
| S-EPMC45850 | biostudies-other
| S-EPMC297615 | biostudies-other
| S-EPMC4092958 | biostudies-literature
| S-EPMC6606986 | biostudies-literature
| S-EPMC1797226 | biostudies-literature
| S-EPMC2909933 | biostudies-literature
| S-EPMC4392269 | biostudies-literature
| S-EPMC5573580 | biostudies-literature
| S-EPMC6140513 | biostudies-literature