Ontology highlight
ABSTRACT:
SUBMITTER: Roberto FF
PROVIDER: S-EPMC54415 | biostudies-other | 1990 Aug
REPOSITORIES: biostudies-other
Roberto F F FF Klee H H White F F Nordeen R R Kosuge T T
Proceedings of the National Academy of Sciences of the United States of America 19900801 15
The gene encoding N epsilon-(indole-3-acetyl)-L-lysine synthetase, iaaL, from Pseudomonas savastanoi was localized within a 4.25-kilobase EcoRI fragment derived from pIAA1 of oleander strain EW 2009. Two open reading frames of 606 and 1188 nucleotides were identified upon sequencing, which directed the in vitro synthesis of Mr 21,000 and Mr 44,000 proteins. Expression of an open reading frame-2 subclone, pMON686, in Escherichia coli indicates that (indole-3-acetyl)-L-lysine synthetase is encoded ...[more]