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Molecular cloning of cDNA for the human tumor-associated antigen CO-029 and identification of related transmembrane antigens.

ABSTRACT: The human tumor-associated antigen CO-029 is a monoclonal antibody-defined cell surface glycoprotein of 27-34 kDa. By using the high-efficiency COS cell expression system, a full-length cDNA clone for CO-029 was isolated. When transiently expressed in COS cells, the cDNA clone directed the synthesis of an antigen reactive to monoclonal antibody CO-029 in mixed hemadsorption and immunoblot assays. Sequence analysis revealed that CO-029 belongs to a family of cell surface antigens that includes the melanoma-associated antigen ME491, the leukocyte cell surface antigen CD37, and the Sm23 antigen of the parasitic helminth Schistosoma mansoni. CO-029 and ME491 antigen expression and the effect of their corresponding monoclonal antibodies on cell growth were compared in human tumor cell lines of various histologic origins.

SUBMITTER: Szala S

PROVIDER: S-EPMC54632 | biostudies-other | 1990 Sep

REPOSITORIES: biostudies-other

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Molecular cloning of cDNA for the human tumor-associated antigen CO-029 and identification of related transmembrane antigens.

Szala S S Kasai Y Y Steplewski Z Z Rodeck U U Koprowski H H Linnenbach A J AJ

Proceedings of the National Academy of Sciences of the United States of America 19900901 17

The human tumor-associated antigen CO-029 is a monoclonal antibody-defined cell surface glycoprotein of 27-34 kDa. By using the high-efficiency COS cell expression system, a full-length cDNA clone for CO-029 was isolated. When transiently expressed in COS cells, the cDNA clone directed the synthesis of an antigen reactive to monoclonal antibody CO-029 in mixed hemadsorption and immunoblot assays. Sequence analysis revealed that CO-029 belongs to a family of cell surface antigens that includes th ...[more]

PMID: 2395876

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