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Antimicrobial Properties of Diamond-Like Carbon/Silver Nanocomposite Thin Films Deposited on Textiles: Towards Smart Bandages.

ABSTRACT: In the current work, a new antibacterial bandage was proposed where diamond-like carbon with silver nanoparticle (DLC:Ag)-coated synthetic silk tissue was used as a building block. The DLC:Ag structure, the dimensions of nanoparticles, the silver concentration and the silver ion release were studied systematically employing scanning electron microscopy, energy dispersive X-ray spectroscopy and atomic absorption spectroscopy, respectively. Antimicrobial properties were investigated using microbiological tests (disk diffusion method and spread-plate technique). The DLC:Ag layer was stabilized on the surface of the bandage using a thin layer of medical grade gelatin and cellulose. Four different strains of Staphylococcus aureus extracted from humans' and animals' infected wounds were used. It is demonstrated that the efficiency of the Ag⁺ ion release to the aqueous media can be increased by further RF oxygen plasma etching of the nanocomposite. It was obtained that the best antibacterial properties were demonstrated by the plasma-processed DLC:Ag layer having a 3.12 at % Ag surface concentration with the dominating linear dimensions of nanoparticles being 23.7 nm. An extra protective layer made from cellulose and gelatin with agar contributed to the accumulation and efficient release of silver ions to the aqueous media, increasing bandage antimicrobial efficiency up to 50% as compared to the single DLC:Ag layer on textile.

SUBMITTER: Juknius T

PROVIDER: S-EPMC5503000 | biostudies-other | 2016 May

REPOSITORIES: biostudies-other

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Project description:A growing number of severe infections are related to antibiotic-resistant bacteria, therefore, in recent years, alternative antimicrobial materials based on silver nanoparticles (NPs) attracted a lot of attention. In the current research, we present a medical patch prototype containing diamond-like carbon nanocomposite thin films doped with silver nanoparticles (DLC:Ag), as a source of silver ions, and an aqueous mass of the gelatin/agar mixture as a silver ion accumulation layer. The DLC:Ag thin films with 3.4 at.% of silver were deposited on synthetic silk employing reactive unbalanced DC magnetron sputtering of the silver target with argon ions performed in the acetylene gas atmosphere. The average size of the silver nanoparticles as defined by scanning electron microscope was 24 nm. After the film deposition, the samples were etched with RF oxygen plasma, aiming at efficient silver ion release in aqueous media from the nanocomposite film. In the patch prototype, a mixture of agar and gelatin was applied in silicone carrier with cavities, acting as a silver ion accumulation layer that further enhanced the antimicrobial efficiency. It was found that the DLC:Ag thin film on the silk after soaking in water for 24 h was able to release up to 4 ppm of Ag. The microbiological experiments using S. aureus bacteria were performed with the patch prototype and the silver ion saturated water, demonstrated the inactivation of 99% and 79% of bacteria, respectively. Scanning electron microscopy analysis showed that silver NPs destroyed the bacteria cell and the bacteria affected by Ag ions had spots and perforated cell wall areas with cytoplasm leakage out was obtained. A preliminary preclinical study using the laboratory animals demonstrated that using the patch prototype, the methicillin-resistant S. aureus (MRSA)-infected wound on skin surface healed faster compared with control and was able to kill all MRSA bacteria strains in the wound's bed after 72 h of treatment.

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Antimicrobial Properties of Diamond-Like Carbon/Silver Nanocomposite Thin Films Deposited on Textiles: Towards Smart Bandages.

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