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Cytotoxicity of Light-Cured Dental Materials according to Different Sample Preparation Methods.

ABSTRACT: Dental light-cured resins can undergo different degrees of polymerization when applied in vivo. When polymerization is incomplete, toxic monomers may be released into the oral cavity. The present study assessed the cytotoxicity of different materials, using sample preparation methods that mirror clinical conditions. Composite and bonding resins were used and divided into four groups according to sample preparation method: uncured; directly cured samples, which were cured after being placed on solidified agar; post-cured samples were polymerized before being placed on agar; and "removed unreacted layer" samples had their oxygen-inhibition layer removed after polymerization. Cytotoxicity was evaluated using an agar diffusion test, MTT assay, and confocal microscopy. Uncured samples were the most cytotoxic, while removed unreacted layer samples were the least cytotoxic (p < 0.05). In the MTT assay, cell viability increased significantly in every group as the concentration of the extracts decreased (p < 0.05). Extracts from post-cured and removed unreacted layer samples of bonding resin were less toxic than post-cured and removed unreacted layer samples of composite resin. Removal of the oxygen-inhibition layer resulted in the lowest cytotoxicity. Clinicians should remove unreacted monomers on the resin surface immediately after restoring teeth with light-curing resin to improve the restoration biocompatibility.

SUBMITTER: Lee MJ

PROVIDER: S-EPMC5503327 | biostudies-other | 2017 Mar

REPOSITORIES: biostudies-other

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Project description:Fabricating method may affect the surface properties and biological characteristics of provisional restorations. This study aimed to evaluate the surface roughness, plaque accumulation, and cytotoxicity of provisional restorative materials fabricated by the conventional, digital subtractive and additive methods. Sixty-six bar-shaped specimens (2×4×10 mm) were fabricated by using provisional restorative materials through the conventional, digital subtractive and additive methods (n = 22 per group). Ten specimens of each group were used for surface roughness and plaque accumulation tests, 10 specimens for cytotoxicity assay, and 2 specimens of each group were used for qualitative assessment by scanning electron microscopy. The Ra (roughness average) and Rz (roughness height) values (μm) were measured via profilometer, and visual inspection was performed through scanning electron microscopy. Plaque accumulation of Streptococcus mutans and cytotoxicity on human gingival fibroblast-like cells were evaluated. The data were analyzed with one-way ANOVA and Tukey's post hoc test (α = 0.05). Surface roughness, biofilm accumulation and cytotoxicity were significantly different among the groups (P<0.05). Surface roughness was significantly higher in the conventional group (P<0.05); however, the two other groups were not significantly different (P>0.05). Significantly higher bacterial attachment was observed in the additive group than the subtractive (P<0.001) and conventional group (P = 0.025); while, the conventional and subtractive groups were statistically similar (P = 0.111). Regarding the cytotoxicity, the additive group had significantly higher cell viability than the subtractive group (P = 0.006); yet, the conventional group was not significantly different from the additive (P = 0.354) and subtractive group (P = 0.101). Surface roughness was the highest in conventionally cured group; but, the additive group had the most plaque accumulation and lowest cytotoxicity.

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Cytotoxicity of Light-Cured Dental Materials according to Different Sample Preparation Methods.

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