Dataset Information

Ageing and latent CMV infection impact on maturation, differentiation and exhaustion profiles of T-cell receptor gammadelta T-cells.

ABSTRACT: Ageing is a broad cellular process, largely affecting the immune system, especially T-lymphocytes. Additionally to immunosenescence alone, cytomegalovirus (CMV) infection is thought to have major impacts on T-cell subset composition and exhaustion. These impacts have been studied extensively in TCR??+ T-cells, with reduction in naive, increase in effector (memory) subsets and shifts in CD4/CD8-ratios, in conjunction with morbidity and mortality in elderly. Effects of both ageing and CMV on the TCR??+ T-cell compartment remain largely elusive. In the current study we investigated V?- and V?-usage, maturation, differentiation and exhaustion marker profiles of both CD4 and CD8 double-negative (DN) and CD8+TCR??+ T-cells in 157 individuals, age range 20-95. We observed a progressive decrease in absolute numbers of total TCR??+ T-cells in blood, affecting the predominant V?9/V?2 population. Aged TCR??+ T-cells appeared to shift from naive to more (late-stage) effector phenotypes, which appeared more prominent in case of persistent CMV infections. In addition, we found effects of both ageing and CMV on the absolute counts of exhausted TCR??+ T-cells. Collectively, our data show a clear impact of ageing and CMV persistence on DN and CD8+TCR??+ T-cells, similar to what has been reported in CD8+TCR??+ T-cells, indicating that they undergo similar ageing processes.

SUBMITTER: Kallemeijn MJ

PROVIDER: S-EPMC5511140 | biostudies-other | 2017 Jul

REPOSITORIES: biostudies-other

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Ageing and latent CMV infection impact on maturation, differentiation and exhaustion profiles of T-cell receptor gammadelta T-cells.

Kallemeijn Martine J MJ Boots Anne Mieke H AMH van der Klift Michèle Y MY Brouwer Elisabeth E Abdulahad Wayel H WH Verhaar Jan A N JAN van Dongen Jacques J M JJM Langerak Anton W AW

Scientific reports 20170714 1

Ageing is a broad cellular process, largely affecting the immune system, especially T-lymphocytes. Additionally to immunosenescence alone, cytomegalovirus (CMV) infection is thought to have major impacts on T-cell subset composition and exhaustion. These impacts have been studied extensively in TCRαβ+ T-cells, with reduction in naive, increase in effector (memory) subsets and shifts in CD4/CD8-ratios, in conjunction with morbidity and mortality in elderly. Effects of both ageing and CMV on the T ...[more]

PMID: 28710491

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Dataset Information

Ageing and latent CMV infection impact on maturation, differentiation and exhaustion profiles of T-cell receptor gammadelta T-cells.

Publications

Ageing and latent CMV infection impact on maturation, differentiation and exhaustion profiles of T-cell receptor gammadelta T-cells.

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