Unknown

Dataset Information

0

Methionine restriction on oxidative stress and immune response in dss-induced colitis mice.


ABSTRACT: A strong correlation exists between inflammatory bowel disease (IBD) and oxidative stress involving alterations of several key signaling pathways. It is known that methionine promotes reactive oxygen species (ROS) production; we therefore hypothesize that a methionine restriction diet would reduce ROS production, inflammatory responses, and the course of IBD. We generated a murine colitis model by dextran sodium sulfate (DSS) treatment and tested the effects of the methionine restriction diet. Forty-eight mice were randomly divided into four groups of equal size, which included a control (CON) group, an MR (methionine restriction diet) group, a DSS treated group and an MR-DSS treated group. Mice in the first two groups had unrestricted access to water for one week. Mice in the two DSS-treated groups had unrestricted access to 5% DSS solution supplied in the drinking water for the same period. Mice in the CON and DSS groups were given a basal diet, whereas mice in the MR-DSS and MR groups were fed a 0.14% MR diet. We found that DSS reduced daily weight gain, suppressed antioxidant enzyme expression, increased histopathology scores and activated NF-κB and nuclear factor erythroid 2-related factor 2/Kelch-like ECH-associated protein 1 (Nrf2/Keap1) signaling. We also showed that the MR diet upregulated catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) activities, decreased myeloperoxidase (MPO), TNF-α and IL-1β, and reversed activation of the NF-κB signaling pathway in MR-DSS mice. Taken together, our results imply that the MR diet may be considered as an adjuvant in IBD therapeutics.

SUBMITTER: Liu G 

PROVIDER: S-EPMC5546498 | biostudies-other | 2017 Jul

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC6815543 | biostudies-literature
| S-EPMC8117093 | biostudies-literature
| S-EPMC10000425 | biostudies-literature
| S-EPMC5478663 | biostudies-literature
| S-EPMC8098793 | biostudies-literature
| S-EPMC8566036 | biostudies-literature
| S-EPMC4059975 | biostudies-literature
| S-EPMC3154603 | biostudies-literature
| S-EPMC9491586 | biostudies-literature
| S-EPMC10104076 | biostudies-literature