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Detection of Isolated Mitochondria-Associated ER Membranes Using the Sigma-1 Receptor.


ABSTRACT: The interface between the endoplasmic reticulum (ER) and mitochondria referred to as the MAM (mitochondria-associated ER membrane) plays important roles in many physiological functions. A specific marker for this important entity of cellular structure is urgently needed. Thus, we propose in this method chapter that the membrane-bound ER chaperone sigma-1 receptor serves as an ideal marker for the MAM. We describe in detail the preparation and purification of the MAM by using the sigma-1 receptor as the marker and demonstrate the uniqueness of this marker by using a variety of cells, peripheral and neuronal.

SUBMITTER: Lewis A 

PROVIDER: S-EPMC5547822 | biostudies-other | 2016

REPOSITORIES: biostudies-other

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