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Structural and Thermal Stability of Polycarbonate Decorated Fumed Silica Nanocomposite via Thermomechanical Analysis and In-situ Temperature Assisted SAXS.

ABSTRACT: The inorganic and organic nanocomposites have enticed wide interest in the field of polymer-based composite systems to augment their physiochemical properties like mechanical strength and electrical conductivity. Achieving interfacial interaction between inorganic filler and polymer matrix is a recurring challenge, which has significant implications for mechanical properties of nanocomposites. In this context, the effect of "interfacial zone" on structural and thermal attributes of the melt blended pristine polycarbonate and polycarbonate (PC) decorated fumed silica nanocomposite have been examined from ambient temperature to the glass transition temperature. Thermomechanical characterization and in-situ temperature assisted small angle X-ray scattering technique (SAXS) were used for contemplating quantitative and qualitative molecular dynamics of developed nanocomposites. Though, the FT-IR spectra have demonstrated some extent of interaction between inorganic and organic groups of composite, the reduced glass transition temperature and storage modulus was ascertained in DMA as well as in DSC, which has been further confirmed by in-situ temperature assisted SAXS. It is envisioned that the utilization of in-situ SAXS in addition to the thermomechanical analysis will render the qualitative and quantitative details about the interfacial zone and its effect on thermal and mechanical properties of nanocomposite at varying temperature conditions.

SUBMITTER: Yadav R

PROVIDER: S-EPMC5550471 | biostudies-other | 2017 Aug

REPOSITORIES: biostudies-other

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Project description:For successful therapeutic interventions in cancer immunotherapy, strong antigen-specific immune responses are required. To this end, immunostimulating cues must be combined with antigens to simultaneously arrive at antigen-presenting cells and initiate cellular immune responses. Recently, imidazoquinolines have shown their vast potential as small molecular Toll-like receptor 7/8 (TLR7/8) agonists for immunostimulation when delivered by nanocarriers. At the same time, peptide antigens are promising antigen candidates but require combination with immune-stimulating adjuvants to boost their immunogenicity and exploit their full potential. Consequently, we herein present biodegradable polycarbonate nanogels as versatile delivery system for adjuvants within the particles' core as well as for peptide antigens by surface decoration. For that purpose, orthogonally addressable multifunctional polycarbonate block copolymers were synthesized, enabling adjuvant conjugation through reactive ester chemistry and peptide decoration by strain-promoted alkyne-azide cycloaddition (SPAAC). In preparation for SPAAC, CD4+-specific peptide sequences of the model protein antigen ovalbumin were equipped with DBCO-moieties by site-selective modification at their N-terminal cysteine. With their azide groups exposed on their surface, the adjuvant-loaded nanogels were then efficiently decorated with DBCO-functional CD4+-peptides by SPAAC. In vitro evaluation of the adjuvant-loaded peptide-decorated gels then confirmed their strong immunostimulating properties as well as their high biocompatibility. Despite their covalent conjugation, the CD4+-peptide-decorated nanogels led to maturation of primary antigen-presenting cells and the downstream priming of CD4+-T cells. Subsequently, the peptide-decorated nanogels loaded with TLR7/8 agonist were successfully processed by antigen-presenting cells, enabling potent immune responses for future application in antigen-specific cancer immunotherapy.

Dataset Information

Structural and Thermal Stability of Polycarbonate Decorated Fumed Silica Nanocomposite via Thermomechanical Analysis and In-situ Temperature Assisted SAXS.

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