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Recombineering using RecET in Corynebacterium glutamicum ATCC14067 via a self-excisable cassette.

ABSTRACT: Gene manipulation is essential for metabolic engineering and synthetic biology, but the current general gene manipulation methods are not applicable to the non-model strain Corynebacterium glutamicum (C. glutamicum) ATCC14067, which is used for amino acid production. Here, we report an effective and sequential deletion method for C. glutamicum ATCC14067 using the exonuclease-recombinase pair RecE?+?RecT (RecET) for recombineering via a designed self-excisable linear double-strand DNA (dsDNA) cassette, which contains the Cre/loxP system, to accomplish markerless deletion. To the best of our knowledge, this is the first effective and simple strategy for recombination with markerless deletion in C. glutamicum ATCC14067. This strategy provides a simple markerless deletion strategy for C. glutamicum and builds a solid basis for producer construction.

SUBMITTER: Huang Y

PROVIDER: S-EPMC5554157 | biostudies-other | 2017 Aug

REPOSITORIES: biostudies-other

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Recombineering using RecET in Corynebacterium glutamicum ATCC14067 via a self-excisable cassette.

Huang Yuanyuan Y Li Lu L Xie Shan S Zhao Nannan N Han Shuangyan S Lin Ying Y Zheng Suiping S

Scientific reports 20170811 1

Gene manipulation is essential for metabolic engineering and synthetic biology, but the current general gene manipulation methods are not applicable to the non-model strain Corynebacterium glutamicum (C. glutamicum) ATCC14067, which is used for amino acid production. Here, we report an effective and sequential deletion method for C. glutamicum ATCC14067 using the exonuclease-recombinase pair RecE + RecT (RecET) for recombineering via a designed self-excisable linear double-strand DNA (dsDNA) cas ...[more]

PMID: 28801604

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Project description:This SuperSeries is composed of the SubSeries listed below.

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Corynebacterium glutamicum ATCC14067

Project description:Not available

2007-11-09 | PRD000026 | Pride

Comprehensive analysis of the Corynebacterium glutamicum transcriptome using an improved RNAseq technique.

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