Early cytoplasmic uncoating is associated with infectivity of HIV-1.
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ABSTRACT:
SUBMITTER: Mamede JI
PROVIDER: S-EPMC5576815 | biostudies-other | 2017 Aug
REPOSITORIES: biostudies-other
Publications
Early cytoplasmic uncoating is associated with infectivity of HIV-1.
Mamede João I JI Cianci Gianguido C GC Anderson Meegan R MR Hope Thomas J TJ
Proceedings of the National Academy of Sciences of the United States of America 20170807 34
After fusion, HIV delivers its conical capsid into the cytoplasm. To release the contained reverse-transcribing viral genome, the capsid must disassemble in a process termed uncoating. Defining the kinetics, dynamics, and cellular location of uncoating of virions leading to infection has been confounded by defective, noninfectious particles and the stochastic minefield blocking access to host DNA. We used live-cell fluorescent imaging of intravirion fluid phase markers to monitor HIV-1 uncoating ...[more]
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