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CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs.

ABSTRACT: Genome editing using programmable nucleases has revolutionized biomedical research. CRISPR-Cas9 mediated zygote genome editing enables high efficient production of knockout animals suitable for studying development and relevant human diseases. Here we report efficient disabling pancreatogenesis in pig embryos via zygotic co-delivery of Cas9 mRNA and dual sgRNAs targeting the PDX1 gene, which when combined with chimeric-competent human pluriopotent stem cells may serve as a suitable platform for the xeno-generation of human tissues and organs in pigs.

SUBMITTER: Wu J

PROVIDER: S-EPMC5585254 | biostudies-other | 2017 Sep

REPOSITORIES: biostudies-other

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CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs.

Wu Jun J Vilarino Marcela M Suzuki Keiichiro K Okamura Daiji D Bogliotti Yanina Soledad YS Park Insung I Rowe Joan J McNabb Bret B Ross Pablo Juan PJ Belmonte Juan Carlos Izpisua JCI

Scientific reports 20170905 1

Genome editing using programmable nucleases has revolutionized biomedical research. CRISPR-Cas9 mediated zygote genome editing enables high efficient production of knockout animals suitable for studying development and relevant human diseases. Here we report efficient disabling pancreatogenesis in pig embryos via zygotic co-delivery of Cas9 mRNA and dual sgRNAs targeting the PDX1 gene, which when combined with chimeric-competent human pluriopotent stem cells may serve as a suitable platform for ...[more]

PMID: 28874671

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