Ontology highlight
ABSTRACT:
SUBMITTER: Yoshina S
PROVIDER: S-EPMC5600330 | biostudies-other | 2016 Mar
REPOSITORIES: biostudies-other
Biochemistry and biophysics reports 20151201
We established a method to generate integration from extrachromosomal arrays with the CRISPR/Cas9 system. Multi-copy transgenes were integrated into the defined loci of chromosomes by this method, while a multi-copy transgene is integrated into random loci by previous methods, such as UV- and gamma-irradiation. The effects of a combination of sgRNAs, which define the cleavage sites in extrachromosomes and chromosomes, and the copy number of potential cleavable sequences were examined. The relati ...[more]