Imaging viscosity of intragranular mucin matrix in cystic fibrosis cells.
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ABSTRACT:
SUBMITTER: Requena S
PROVIDER: S-EPMC5711894 | biostudies-other | 2017 Dec
REPOSITORIES: biostudies-other
Publications
Imaging viscosity of intragranular mucin matrix in cystic fibrosis cells.
Requena Sebastian S Ponomarchuk Olga O Castillo Marlius M Rebik Jonathan J Brochiero Emmanuelle E Borejdo Julian J Gryczynski Ignacy I Dzyuba Sergei V SV Gryczynski Zygmunt Z Grygorczyk Ryszard R Fudala Rafal R
Scientific reports 20171201 1
Abnormalities of mucus viscosity play a critical role in the pathogenesis of several respiratory diseases, including cystic fibrosis. Currently, there are no approaches to assess the rheological properties of mucin granule matrices in live cells. This is the first example of the use of a molecular rotor, a BODIPY dye, to quantitatively visualize the viscosity of intragranular mucin matrices in a large population of individual granules in differentiated primary bronchial epithelial cells using fl ...[more]
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