Ontology highlight
ABSTRACT:
SUBMITTER: Miyazaki T
PROVIDER: S-EPMC5725452 | biostudies-other | 2017 Dec
REPOSITORIES: biostudies-other
Miyazaki Takatsugu T Ishizaki Masaaki M Dohra Hideo H Park Sungjo S Terzic Andre A Kato Tatsuya T Kohsaka Tetsuya T Park Enoch Y EY
Scientific reports 20171211 1
Insulin-like peptide 3 (INSL3) is a member of the relaxin/insulin superfamily and is expressed in testicular Leydig cells. Essential for fetal testis descent, INSL3 has been implicated in testicular and sperm function in adult males via interaction with relaxin/insulin-like family peptide receptor 2 (RXFP2). The INSL3 is typically prepared using chemical synthesis or overexpression in Escherichia coli followed by oxidative refolding and proteolysis. Here, we expressed and purified full-length po ...[more]