Dataset Information

OsPT4 Contributes to Arsenate Uptake and Transport in Rice.

ABSTRACT: Arsenic (As) is toxic to organisms, and elevated As accumulation in rice (Oryza sativa) grain may pose a significant health risk to humans. The predominant form of As in soil under aerobic conditions is As(V), which has a chemical structure similar to that of PO43-. Rice roots take up As(V) by phosphate (Pi) transporters, such as OsPT1 and OsPT8. In the present study, we investigated the contribution of OsPT4, belonging to the Pht1 family, on rice As(V) uptake and transport. We determined the mRNA amounts of OsPTs in rice seedlings, and expressions of OsPT1, OsPT4, and OsPT8 were up-regulated under As(V) conditions. OsPT4-overexpressing plants were obtained to examine the As (V) transport activity of OsPT4 in rice. When transgenic rice grew in hydroponic culture with 25 and 50 ?M As(V), the plants showed sensitivity to As(V) stress with aboveground parts showing delayed growth and the roots stunted. The OsPT4 CRISPR lines showed the opposite phenotype. When plants were grown in 5 ?M As(V) solution for 7 days, the As accumulation of OsPT4-overexpressing plants increased up to twice in roots and shoots. Furthermore, the arsenate uptake rates of OsPT4-overexpressing lines were higher compared with wild type. The Vmax of As(V) uptake in OsPT4-overexpressing plants increased 23-45% compared with Nipponbare. In the flooded soil, the As accumulation of OsPT4-overexpressing plants increased 40-66% and 22-30% in straw and grain, respectively. While in OsPT4-cr plants As accumulation in roots decreased 17-30% compared with Nipponbare. Therefore, the present study indicates that OsPT4 is involved in As(V) uptake and transport and could be a good candidate gene to generate low As-accumulating rice.

SUBMITTER: Ye Y

PROVIDER: S-EPMC5744437 | biostudies-other | 2017

REPOSITORIES: biostudies-other

ACCESS DATA

Publications

OsPT4 Contributes to Arsenate Uptake and Transport in Rice.

Ye Ying Y Li Peng P Xu Tangqian T Zeng Liting L Cheng Deng D Yang Meng M Luo Jie J Lian Xingming X

Frontiers in plant science 20171222

Arsenic (As) is toxic to organisms, and elevated As accumulation in rice (Oryza sativa) grain may pose a significant health risk to humans. The predominant form of As in soil under aerobic conditions is As(V), which has a chemical structure similar to that of PO43-. Rice roots take up As(V) by phosphate (Pi) transporters, such as OsPT1 and OsPT8. In the present study, we investigated the contribution of OsPT4, belonging to the Pht1 family, on rice As(V) uptake ...[more]

PMID: 29312424

Similar Datasets

Project description:BackgroundGenes associated with arsenite uptake and transport in rice plants (i.e., OsLsi1, OsLsi2, OsLsi3, OsLsi6 and OsABCC1) have been identified to date. However, their expression over time during the whole growth period of rice under arsenite stress conditions is still poorly understood. In this study, the dynamics of gene expression associated with arsenite transport and arsenic concentrations in different organs of rice were investigated to determine the critical period(s) of arsenite uptake and translocation regulated by gene expression during the whole growth period.ResultsThe relative expression of OsLsi2 and OsLsi1 in the roots was upregulated and reached its highest value (2-∆∆Ct = 4.04 and 1.19, respectively) at the jointing stage (9 weeks after transplantation), in which the arsenic concentration in roots also was the highest at 144 mg/kg. A range from 45.1 to 61.2% of total arsenic accumulated in the roots during seedling to heading stages (3-16 weeks), which was mainly associated with the relatively high expression of OsABCC1 (1.50-7.68), resulting in arsenic located in the vacuoles of roots. Subsequently, the As translocation factor from root to shoot increased over time from heading to milky ripe (16-20 weeks), and 74.3% of the arsenic accumulated in shoots at the milk stage. Such an increase in arsenic accumulation in shoots was likely related to the findings that (i) OsABCC1 expression in roots was suppressed to 0.14-0.75 in 18-20 weeks; (ii) OsLsi3 and OsABCC1 expression in nodes I, II, and III was upregulated to 4.01-25.8 and 1.59-2.36, respectively, in 16-20 weeks; and (iii) OsLsi6 and OsABCC1 expression in leaves and husks was significantly upregulated to 2.03-5.26 at 18 weeks.ConclusionsThe jointing stage is the key period for the expression of arsenite-transporting genes in roots, and the heading to milky ripe stages are the key period for the expression of arsenite-transporting genes in shoots, both of which should be considered for regulation during safe rice production in arsenic-contaminated paddy soil.

Dataset Information

OsPT4 Contributes to Arsenate Uptake and Transport in Rice.

Publications

<i>OsPT4</i> Contributes to Arsenate Uptake and Transport in Rice.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets