Unknown

Dataset Information

0

Sde2 is an intron-specific pre-mRNA splicing regulator activated by ubiquitin-like processing.


ABSTRACT: The expression of intron-containing genes in eukaryotes requires generation of protein-coding messenger RNAs (mRNAs) via RNA splicing, whereby the spliceosome removes non-coding introns from pre-mRNAs and joins exons. Spliceosomes must ensure accurate removal of highly diverse introns. We show that Sde2 is a ubiquitin-fold-containing splicing regulator that supports splicing of selected pre-mRNAs in an intron-specific manner in Schizosaccharomyces pombe Both fission yeast and human Sde2 are translated as inactive precursor proteins harbouring the ubiquitin-fold domain linked through an invariant GGKGG motif to a C-terminal domain (referred to as Sde2-C). Precursor processing after the first di-glycine motif by the ubiquitin-specific proteases Ubp5 and Ubp15 generates a short-lived activated Sde2-C fragment with an N-terminal lysine residue, which subsequently gets incorporated into spliceosomes. Absence of Sde2 or defects in Sde2 activation both result in inefficient excision of selected introns from a subset of pre-mRNAs. Sde2 facilitates spliceosomal association of Cactin/Cay1, with a functional link between Sde2 and Cactin further supported by genetic interactions and pre-mRNA splicing assays. These findings suggest that ubiquitin-like processing of Sde2 into a short-lived activated form may function as a checkpoint to ensure proper splicing of certain pre-mRNAs in fission yeast.

SUBMITTER: Thakran P 

PROVIDER: S-EPMC5753039 | biostudies-other | 2018 Jan

REPOSITORIES: biostudies-other

altmetric image

Publications

Sde2 is an intron-specific pre-mRNA splicing regulator activated by ubiquitin-like processing.

Thakran Poonam P   Pandit Prashant Arun PA   Datta Sumanjit S   Kolathur Kiran Kumar KK   Pleiss Jeffrey A JA   Mishra Shravan Kumar SK  

The EMBO journal 20170925 1


The expression of intron-containing genes in eukaryotes requires generation of protein-coding messenger RNAs (mRNAs) via RNA splicing, whereby the spliceosome removes non-coding introns from pre-mRNAs and joins exons. Spliceosomes must ensure accurate removal of highly diverse introns. We show that Sde2 is a ubiquitin-fold-containing splicing regulator that supports splicing of selected pre-mRNAs in an intron-specific manner in <i>Schizosaccharomyces pombe</i> Both fission yeast and human Sde2 a  ...[more]

Similar Datasets

| S-EPMC5207164 | biostudies-literature
2024-10-14 | GSE277952 | GEO
| S-EPMC8355544 | biostudies-literature
2024-10-16 | GSE277949 | GEO
| S-EPMC4109538 | biostudies-literature
| S-EPMC5932401 | biostudies-literature
2024-10-16 | GSE277951 | GEO
2024-10-16 | GSE277950 | GEO
| S-EPMC361031 | biostudies-other
| S-EPMC7615767 | biostudies-literature