Other

Dataset Information

0

SFSWAP is a negative regulator of OGT intron detention and global pre-mRNA splicing [CRISPR]


ABSTRACT: In this study, we identified SFSWAP as a negative regulator of O-GlcNAc transferase (OGT) intron 4 detention and global pre-mRNA splicing. To do this, we performed CRISPR knockout screens using the Brunello knockout library on Thiamet G (TG)-treated HCT116 cells containing an O-GlcNAc responsive GFP reporter (GFP-β-OGT) integrated at the AAVS1 locus. Knockout of negative regulatory factors which regulate OGT intron 4 detention lead to increased GFP expression. Using replicate screens and further biochemical validation, we identified SFSWAP as one of the top hits affecting OGT intron 4 detention.

ORGANISM(S): Homo sapiens

PROVIDER: GSE277949 | GEO | 2024/10/16

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2024-10-16 | GSE277950 | GEO
2024-10-16 | GSE277951 | GEO
2021-05-04 | GSE172217 | GEO
2019-11-04 | GSE134993 | GEO
| PRJNA665731 | ENA
2021-11-01 | PXD028141 | Pride
2013-02-07 | E-GEOD-36620 | biostudies-arrayexpress
2017-06-27 | GSE100473 | GEO
2020-04-03 | GSE138783 | GEO
2021-09-08 | PXD016041 | Pride