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Electroporation-delivered fluorescent protein biosensors for probing molecular activities in cells without genetic encoding.

ABSTRACT: Fluorescent protein biosensors are typically implemented via genetic encoding which makes the examination of scarce cell samples impractical. By directly delivering the protein form of the biosensor into cells using electroporation, we detected intracellular molecular activity with the sample size down to ?100 cells with high spatiotemporal resolution.

SUBMITTER: Sun C

PROVIDER: S-EPMC5783554 | biostudies-other | 2014 Oct

REPOSITORIES: biostudies-other

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Electroporation-delivered fluorescent protein biosensors for probing molecular activities in cells without genetic encoding.

Sun Chen C Ouyang Mingxing M Cao Zhenning Z Ma Sai S Alqublan Hamzeh H Sriranganathan Nammalwar N Wang Yingxiao Y Lu Chang C

Chemical communications (Cambridge, England) 20141001 78

Fluorescent protein biosensors are typically implemented via genetic encoding which makes the examination of scarce cell samples impractical. By directly delivering the protein form of the biosensor into cells using electroporation, we detected intracellular molecular activity with the sample size down to ∼100 cells with high spatiotemporal resolution. ...[more]

PMID: 25133322

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