Biochemical Effects of Exercise on a Fasciocutaneous Flap in a Rat Model.

ABSTRACT: An overwhelming amount of data suggest that cardiovascular exercise has a positive effect on the mind and body, although the precise mechanism is not always clear.To assess the clinical and biochemical effects of voluntary cardiovascular exercise on pedicled flaps in a rodent model.Eighteen adult Sprague-Dawley male rats were randomized into a resting animal group (RAG) (n=9) and an exercise animal group (EAG) (n=9) for 14 days (July 23, 2013, through July 30, 2013). A pedicled transposition flap was performed on the ventral surface of the rat, and biopsy specimens were taken from the proximal, middle, and distal portions on postoperative days 0, 2, 5, and 9. Flap survival was analyzed planimetrically, and biopsy specimens were analyzed by hematoxylin-eosin-stained microscopy and immunoblotting. The housing, exercise, surgery, and analysis of the rats were conducted at a single basic science research laboratory at the tertiary care center campus of Thomas Jefferson University in Philadelphia, Pennsylvania.The rats were caged for 14 days or housed in a cage connected to an exercise wheel and pedometer.Study measures were gross and micrographic necrosis and expression of proteins within cell survival and apoptosis pathways.A total of 18 rats were studied, 9 in the RAG and 9 in the EAG. the mean (SEM) amount of necrosis in flaps was 41.3% (3%) in the RAG rats and 10.5% (3.5%) in the EAG rats (P?

SUBMITTER: Aksamitiene E 

PROVIDER: S-EPMC5815117 | biostudies-other | 2017 Jul

REPOSITORIES: biostudies-other