Ontology highlight
ABSTRACT:
SUBMITTER: Ohtsuka M
PROVIDER: S-EPMC5828090 | biostudies-other | 2018 Feb
REPOSITORIES: biostudies-other
Ohtsuka Masato M Sato Masahiro M Miura Hiromi H Takabayashi Shuji S Matsuyama Makoto M Koyano Takayuki T Arifin Naomi N Nakamura Shingo S Wada Kenta K Gurumurthy Channabasavaiah B CB
Genome biology 20180226 1
We present a robust method called improved-Genome editing via Oviductal Nucleic Acids Delivery (i-GONAD) that delivers CRISPR ribonucleoproteins to E0.7 embryos via in situ electroporation. The method generates mouse models containing single-base changes, kilobase-sized deletions, and knock-ins. The efficiency of i-GONAD is comparable to that of traditional microinjection methods, which rely on ex vivo handling of zygotes and require recipient animals for embryo transfer. In contrast, i-GONAD av ...[more]