Dataset Information

Efficient detection of Zika virus RNA in patients' blood from the 2016 outbreak in Campinas, Brazil.

ABSTRACT: Infection with Zika virus (ZIKV), a mosquito-borne flavivirus has been casually linked with increased congenital microcephaly in Brazil from 2015 through 2016. Sensitive and specific diagnosis of patients with Zika fever (ZIKF) remains critical for patient management. We developed a ZIKV NS5 qRT-PCR assay by combining primers described by Balm et al. and a new Taqman probe. The assay was evaluated and compared with another assay described by Lanciotti et al. (ZIKV 1107) using 51 blood and 42 urine samples from 54 suspected ZIKV patients. ZIKV NS5 performed better in terms of sensitivity with more samples detected as ZIKV-positive (n?=?37) than ZIKV 1107 (n?=?34) for urine, and ZIKV-positive (n?=?29) than ZIKV 1107 (n?=?26) for blood. Both assays displayed good overall agreement for urine (?appa?=?0.770) and blood (?appa?=?0.825) samples. Improved availability of validated diagnostic tests, such ZIKV NS5 qRT-PCR, will be critical to ensure adequate and accurate ZIKV diagnosis.

SUBMITTER: Judice CC

PROVIDER: S-EPMC5838246 | biostudies-other | 2018 Mar

REPOSITORIES: biostudies-other

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Efficient detection of Zika virus RNA in patients' blood from the 2016 outbreak in Campinas, Brazil.

Judice Carla Cristina CC Tan Jeslin J L JJL Parise Pierina Lorencini PL Kam Yiu-Wing YW Milanez Guilherme Paier GP Leite Juliana Almeida JA Caserta Leonardo Cardia LC Arns Clarice Weis CW Resende Mariangela Ribeiro MR Angerami Rodrigo R Amaral Eliana E Junior Renato Passini RP Freitas André Ricardo Ribas ARR Costa Fabio Trindade Maranhão FTM Proenca-Modena Jose Luiz JL Ng Lisa F P LFP

Scientific reports 20180305 1

Infection with Zika virus (ZIKV), a mosquito-borne flavivirus has been casually linked with increased congenital microcephaly in Brazil from 2015 through 2016. Sensitive and specific diagnosis of patients with Zika fever (ZIKF) remains critical for patient management. We developed a ZIKV NS5 qRT-PCR assay by combining primers described by Balm et al. and a new Taqman probe. The assay was evaluated and compared with another assay described by Lanciotti et al. (ZIKV 1107) using 51 blood and 42 uri ...[more]

PMID: 29507368

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Project description:ObjectiveDuring the 2016-2017 Zika virus (ZIKV) outbreak, the prevention of unintended pregnancies was identified as a primary strategy to prevent birth defects. This study estimated the cost-effectiveness of the Zika Contraception Access Network (Z-CAN), an emergency response intervention that provided women in Puerto Rico with access to the full range of reversible contraception at no cost and compared results with a preimplementation hypothetical cost-effectiveness analysis (CEA).Study designWe evaluated costs and outcomes of Z-CAN from a health sector perspective compared to no intervention using a decision tree model. Number of people served, contraception methods mix, and costs under Z-CAN were from actual program data and other input parameters were from the literature. Health outcome measures included the number of Zika-associated microcephaly (ZAM) cases and unintended pregnancies. The economic benefits of the Z-CAN intervention were ZIKV-associated direct costs avoided, including lifetime medical and supportive costs associated with ZAM cases, costs of monitoring ZIKV-exposed pregnancies and infants born from Zika-virus infected mothers, and the costs of unintended pregnancies prevented during the outbreak as a result of increased contraception use through the Z-CAN intervention.ResultsThe Z-CAN intervention cost a total of $26.1 million, including costs for the full range of reversible contraceptive methods, contraception related services, and programmatic activities. The program is estimated to have prevented 85% of cases of estimated ZAM cases and unintended pregnancies in the absence of Z-CAN. The intervention cost was projected to have been more than offset by $79.9 million in ZIKV-associated costs avoided, 96% of which were lifetime ZAM-associated costs, as well as $137.0 million from avoided unintended pregnancies, with total net savings in one year of $216.9 million. The results were consistent with the previous CEA study.ConclusionZ-CAN was likely cost-saving in the context of a public health emergency response setting.

Dataset Information

Efficient detection of Zika virus RNA in patients' blood from the 2016 outbreak in Campinas, Brazil.

Publications

Efficient detection of Zika virus RNA in patients' blood from the 2016 outbreak in Campinas, Brazil.

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