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Juglans regia L. protects against UVB induced apoptosis in human epidermal keratinocytes.

ABSTRACT: The present study was aimed to investigate the photoprotective effect of the male flower of J. regia L. (MEJR) against ultraviolet-B induced apoptosis in human skin cells. Human skin epidermal keratinocytes were pretreated with the MEJR (80 µg/ml, has been selected after MTT assay), prior to 30 min UVB-irradiation at a dose of 20 mJ/cm2. Mitochondrial membrane potential was evaluated using Rhodamine-123 staining; the % apoptosis by Hoechst staining and acridine orange staining; DNA damage was measured by comet assay. The levels of p53, Bax, Bcl-xL, Bcl-2, Cytochrome c, Caspase-9 and Caspase-3 expression in HaCaT cells were analyzed by western blotting and RT-PCR. Pretreatment with MEJR 80 µg/ml prior to UVB-irradiation significantly prevents apoptotic characteristics, DNA damage and loss of mitochondrial membrane potential. Thus, MEJR protects UVB-mediated human skin cells, by modulating the expression of apoptotic markers and UVB-induced DNA damage in HaCaT cells.

SUBMITTER: Muzaffer U 

PROVIDER: S-EPMC5857159 | biostudies-other | 2018 Mar

REPOSITORIES: biostudies-other

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<i>Juglans regia L</i>. protects against UVB induced apoptosis in human epidermal keratinocytes.

Muzaffer Umar U   Paul V I VI   Prasad Nagarajan Rajendra NR   Karthikeyan Ramasamy R  

Biochemistry and biophysics reports 20180128

The present study was aimed to investigate the photoprotective effect of the male flower of <i>J. regia</i> L. (MEJR) against ultraviolet-B induced apoptosis in human skin cells. Human skin epidermal keratinocytes were pretreated with the MEJR (80 µg/ml, has been selected after MTT assay), prior to 30 min UVB-irradiation at a dose of 20 mJ/cm<sup>2</sup>. Mitochondrial membrane potential was evaluated using Rhodamine-123 staining; the % apoptosis by Hoechst staining and acridine orange staining;  ...[more]

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