Ontology highlight
ABSTRACT:
SUBMITTER: Sherrard A
PROVIDER: S-EPMC5898265 | biostudies-other | 2018 Mar
REPOSITORIES: biostudies-other
Biology open 20180323 3
Changes in chromatin structure are key determinants of genomic responses. Thus, methods that enable such measurements are instrumental for investigating genome regulation and function. Here, we report further developments and validation of a streamlined method of histone-based fluorescence lifetime imaging microscopy (FLIM) that robustly detects chromatin compaction states in fixed and live cells, in 2D and 3D. We present a quality-controlled and detailed method that is simpler and faster than p ...[more]