Dataset Information

Efficient gene transfection to the brain with ultrasound irradiation in mice using stabilized bubble lipopolyplexes prepared by the surface charge regulation method.

ABSTRACT: We previously developed anionic ternary bubble lipopolyplexes, an ultrasound-responsive carrier, expecting safe and efficient gene transfection. However, bubble lipopolyplexes have a low capacity for echo gas (C3F8) encapsulation (EGE) in nonionic solution such as 5% glucose. On the other hand, we were able to prepare bubble lipopolyplexes by inserting phosphate-buffered saline before C3F8 encapsulation. Surface charge regulation (SCR) by electrolytes stabilizes liposome/plasmid DNA (pDNA) complexes by accelerated membrane fusion. Considering these facts, we hypothesized that SCR by electrolytes such as NaCl would promote C3F8 encapsulation in bubble lipopolyplexes mediated by accelerated membrane fusion. We defined this hypothesis as SCR-based EGE (SCR-EGE). Bubble lipopolyplexes prepared by the SCR-EGE method (SCR-EGE bubble lipopolyplexes) are expected to facilitate the gene transfection because of the high amount of C3F8. Therefore, we applied these methods for gene delivery to the brain and evaluated the characteristics of transgene expression in the brain.First, we measured the encapsulation efficiency of C3F8 in SCR-EGE bubble lipopolyplexes. Next, we applied these bubble lipopolyplexes to the mouse brain; then, we evaluated the transfection efficiency. Furthermore, three-dimensional transgene distribution was observed using multicolor deep imaging.SCR-EGE bubble lipopolyplexes had a higher C3F8 content than conventional bubble lipopolyplexes. In terms of safety, SCR-EGE bubble lipopolyplexes possessed an anionic potential and showed no aggregation with erythrocytes. After applying SCR-EGE bubble lipopolyplexes to the brain, high transgene expression was observed by combining with ultrasound irradiation. As a result, transgene expression mediated by SCR-EGE bubble lipopolyplexes was observed mainly on blood vessels and partially outside of blood vessels.The SCR-EGE method may promote C3F8 encapsulation in bubble lipopolyplexes, and SCR-EGE bubble lipopolyplexes may be potent carriers for efficient and safe gene transfection in the brain, especially to the blood vessels.

SUBMITTER: Ogawa K

PROVIDER: S-EPMC5907898 | biostudies-other | 2018

REPOSITORIES: biostudies-other

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Efficient gene transfection to the brain with ultrasound irradiation in mice using stabilized bubble lipopolyplexes prepared by the surface charge regulation method.

Ogawa Koki K Fuchigami Yuki Y Hagimori Masayori M Fumoto Shintaro S Miura Yusuke Y Kawakami Shigeru S

International journal of nanomedicine 20180416

<h4>Introduction</h4>We previously developed anionic ternary bubble lipopolyplexes, an ultrasound-responsive carrier, expecting safe and efficient gene transfection. However, bubble lipopolyplexes have a low capacity for echo gas (C<sub>3</sub>F<sub>8</sub>) encapsulation (EGE) in nonionic solution such as 5% glucose. On the other hand, we were able to prepare bubble lipopolyplexes by inserting phosphate-buffered saline before C<sub>3</sub>F<sub>8</sub> encapsulation. Surface charge regulation ( ...[more]

PMID: 29713163

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Project description:One of the significant issues persisting in the study of soil stabilization is the establishment of the optimum proportions of the quantity of stabilizer to be added to the soil. Determining optimum solutions or the most feasible remedies for the utilization of stabilizing products in terms of their dose rates has become a significant concern in major civil engineering design projects. Using the response surface methodology, this study primarily focused on investigating the optimal levels of reinforcement fiber length (FL), fiber dosage (FD), and curing time (CT) for geotechnical parameters of stabilized soil. To realize this objective, an experimental study was undertaken on the California bearing ratio (CBR) and unconfined compressive strength (UCS). Hydraulic conductivity (HC) tests were also performed, with stabilizer proportions of 6-12 mm for the FL and 0.2-0.6% for the FD calculated for the total dry weight of soil and 6% lime (total weight of dry soil). The curing times used for testing were 0, 7, and 14 days for the CBR tests; 60, 210, and 360 days for the UCS tests; and 7, 17, and 28 days for the HC tests. All practical experiments were conducted with experimental techniques using stabilizer proportions and curing times. The FL, FD, CT, CBR, UCS, and HC response factors were determined using the central composite design. The results point toward a statistically significant model constructed (p ≤ 0.05) using the analysis of variance. The results from this optimization procedure show that the optimal values for the FL, FD, and CT were 11.1 mm, 0.5%, and 13.2 days, respectively, as these provided the maximum values for the CBR; 11.7 mm for the FL, 0.3% for the FD, and 160 days for the CT corresponded to the maximum values for the UCS; and 10.5 mm for the FL, 0.5% for the FD, and 15 days for the CT led to the minimum value for the HC. In practice, the suggested values may be useful for experiments, especially for preliminary assessments prior to stabilization.

Dataset Information

Efficient gene transfection to the brain with ultrasound irradiation in mice using stabilized bubble lipopolyplexes prepared by the surface charge regulation method.

Publications

Efficient gene transfection to the brain with ultrasound irradiation in mice using stabilized bubble lipopolyplexes prepared by the surface charge regulation method.

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