Unraveling the Inconsistencies of Cardiac Differentiation Efficiency Induced by the GSK3? Inhibitor CHIR99021 in Human Pluripotent Stem Cells.
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ABSTRACT:
SUBMITTER: Laco F
PROVIDER: S-EPMC5989659 | biostudies-other | 2018 Jun
REPOSITORIES: biostudies-other
Publications
Unraveling the Inconsistencies of Cardiac Differentiation Efficiency Induced by the GSK3β Inhibitor CHIR99021 in Human Pluripotent Stem Cells.
Laco Filip F Woo Tsung Liang TL Zhong Qixing Q Szmyd Radoslaw R Ting Sherwin S Khan Fahima Jaleel FJ Chai Christina L L CLL Reuveny Shaul S Chen Allen A Oh Steve S
Stem cell reports 20180426 6
Cardiac differentiation efficiency is hampered by inconsistencies and low reproducibility. We analyzed the differentiation process of multiple human pluripotent stem cell (hPSC) lines in response to dynamic GSK3β inhibition under varying cell culture conditions. hPSCs showed strong differences in cell-cycle profiles with varying culture confluency. hPSCs with a higher percentage of cells in the G1 phase of the cell cycle exhibited cell death and required lower doses of GSK3β inhibitors to induce ...[more]
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