Ontology highlight
ABSTRACT:
SUBMITTER: Sano M
PROVIDER: S-EPMC6187859 | biostudies-other | 2018 Apr
REPOSITORIES: biostudies-other
Sano Mamiko M Kaji Noritada N Wu Qiong Q Naito Toyohiro T Yasui Takao T Taniguchi Masateru M Kawai Tomoji T Baba Yoshinobu Y
Micromachines 20180413 4
In the present study, we quantitatively evaluated dielectric breakdown in silicon-based micro- and nanofluidic devices under practical electrophoretic conditions by changing the thickness of the insulating layer. At higher buffer concentration, a silicon nanofluidic device with a 100 nm or 250 nm silicon dioxide layer tolerated dielectric breakdown up to ca. 10 V/cm, thereby allowing successful electrophoretic migration of a single DNA molecule through a nanochannel. The observed DNA migration b ...[more]