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Electrophoretic Separations on Parafilm-Paper-Based Analytical Devices.

ABSTRACT: Microfluidic paper-based analytical devices (mPADs) have gained significant attention in recent years for applications ranging from clinical diagnostics to environmental testing. However, separation on mPADs remain challenging to implement, particularly in complex samples. This has revived interest in revisiting paper chromatography and paper electrophoresis in mPADs to address these needs. Here, laminated Parafilm-paper (l-paper) is applied to fabricate electrophoretic devices. This approach yields a free-standing channel, leading to improved peak resolution relative to previous electrophoretic separations in traditional wax-printed mPADs. Major factors influencing the separation, including Joule heating, electroosmotic flow, and electrophoretic mobility, were investigated. As a result of paper's high ratio of surface area (78%) to pore volume (22%) resulting in slow heat dissipation, a usable applied field strength range of 0 - 200 V cm-1 was employed to avoid Joule heating. The electroosmotic flow of the system was found to be 2.5 × 10-5 ± 7.7 × 10-7 cm2 V-1s-1 and the electrophoretic mobility of chlorophenol red was 1.2 × 10-4 ± 7.7 × 10-7 cm2 V-1s-1. Basic separation protocols were optimized using colorimetric detection of chlorophenol red and indigo carmine dyes as representative molecules. Paper type, channel width, and applied potential were then used to optimize the separations. Addition of an injection port to the device improved resolution and reduced peak broadening. Finally, the separation of fluorescein isothiocyanate (FITC) and L-glutamic acid (Glu) labeled with FITC, was successfully carried out using the l-paper electrophoretic device. Imaging with a microscope was found to achieve reduced peak broadening and increased resolution relative to imaging with a mobile camera, due to elimination of background signal, achieving a 72 ± 4% conjugation of Glu and FITC.

SUBMITTER: Mettakoonpitak J

PROVIDER: S-EPMC7450514 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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Electrophoretic Separations on Parafilm-Paper-Based Analytical Devices.

Mettakoonpitak Jaruwan J Henry Charles S CS

Sensors and actuators. B, Chemical 20180630

Microfluidic paper-based analytical devices (mPADs) have gained significant attention in recent years for applications ranging from clinical diagnostics to environmental testing. However, separation on mPADs remain challenging to implement, particularly in complex samples. This has revived interest in revisiting paper chromatography and paper electrophoresis in mPADs to address these needs. Here, laminated Parafilm-paper (l-paper) is applied to fabricate electrophoretic devices. This approach yi ...[more]

PMID: 32863586

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Project description:Numerous fabrication methods have been reported for microfluidic paper-based analytical devices (μPADs) using barrier materials ranging from photoresist to wax. While these methods have been used with wide success, consistently producing small, high-resolution features using materials and methods that are compatible with solvents and surfactants remains a challenge. Two new methods are presented here for generating μPADs with well-defined, high-resolution structures compatible with solvents and surfactant-containing solutions by partially or fully fusing paper with Parafilm® followed by cutting with a CO2 laser cutter. Partial fusion leads to laminated paper (l-paper) while the complete fusion results in infused paper (i-paper). Patterned structures in l-paper were fabricated by selective removal of the paper but not the underlying Parafilm® using a benchtop CO2 laser. Under optimized conditions, a gap as small as 137 ± 22 μm could be generated. Using this approach, a miniaturized paper 384-zone plate, consisting of circular detection elements with a diameter of 1.86 mm, was fabricated in 64 × 43 mm2 area. Furthermore, these ablation-patterned substrates were confirmed to be compatible with surfactant solutions and common organic solvents (methanol, acetonitrile and dimethylformamide), which has been achieved by very few μPAD patterning techniques. Patterns in i-paper were created by completely cutting out zones of the i-paper and then fixing pre-cut paper into these openings similar to the strategy of fitting a jigsaw piece into a puzzle. Upon heating, unmodified paper was readily sealed into these openings due to partial reflow of the paraffin into the paper. This unique and simple bonding method was illustrated by two types of 3D μPADs, a push-on valve and a time-gated flow distributor, without adding adhesive layers. The free-standing jigsaw-patterned sheets showed good structural stability and solution compatibility, which provided a facile alternative method for fabricating complicated μPADs.

Dataset Information

Electrophoretic Separations on Parafilm-Paper-Based Analytical Devices.

Publications

Electrophoretic Separations on Parafilm-Paper-Based Analytical Devices.

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