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Adipose tissue: A natural resource for multipotent mesenchymal stem cells with potential translation to trigerminal layers.

ABSTRACT: The article reports basic science research that establishes that adipose tissue (AT)-derived mesenchymal stem cells (MSCs) have a potential to transgerminal translation. MSC confirmation was obtained by phenotypic spindle-shaped cells as well as with four positive and three negative markers. The translineage translation of adipose-derived MSCs (ADMSCs) was established. The lipoaspirate was subjected to enzymatic digestion with collagenase. Stromal vascular factor (SVF) was isolated. With two passages, pure culture of ADMSCs was obtained. They were translated to all the three germinal layers. AT-derived SVF contains ~30% MSCs. They are capable of being translated into endoderm, mesoderm and ectoderm. AT is a rich source for MSCs, with immense research possibilities for regeneration and rejuvenation.

SUBMITTER: Vyas B

PROVIDER: S-EPMC6219375 | biostudies-other | 2018 May-Aug

REPOSITORIES: biostudies-other

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Adipose tissue: A natural resource for multipotent mesenchymal stem cells with potential translation to trigerminal layers.

Vyas Bhaskar B Shah Atul A Marathe Anant A Ansarullah Vyas Rajni R Bhonde Ramesh R

Indian journal of plastic surgery : official publication of the Association of Plastic Surgeons of India 20180501 2

<h4>Background</h4>The article reports basic science research that establishes that adipose tissue (AT)-derived mesenchymal stem cells (MSCs) have a potential to transgerminal translation.<h4>Study design</h4>MSC confirmation was obtained by phenotypic spindle-shaped cells as well as with four positive and three negative markers. The translineage translation of adipose-derived MSCs (ADMSCs) was established.<h4>Materials and methods</h4>The lipoaspirate was subjected to enzymatic digestion with c ...[more]

PMID: 30505088

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Project description:Rotator cuff injuries are a common clinical problem either as a result of overuse or aging. Biological approaches to tendon repair that involve use of scaffolding materials or cell-based approaches are currently being investigated. The cell-based approaches are focused on applying multipotent mesenchymal stem cells (MSCs) mostly harvested from bone marrow. In the present study, we focused on characterizing cells harvested from tissues associated with rotator cuff tendons based on an assumption that these cells would be more appropriate for tendon repair. We isolated MSCs from bursa tissue associated with rotator cuff tendons and characterized them for multilineage differentiation in vitro and in vivo. Human bursa was obtained from patients undergoing rotator cuff surgery and cells within were isolated using collagenase and dispase digestion. The cells isolated from the tissues were characterized for osteoblastic, adipogenic, chondrogenic, and tenogenic differentiation in vitro and in vivo. The results showed that the cells isolated from bursa tissue exhibited MSCs characteristics as evidenced by the expression of putative cell surface markers attributed to MSCs. The cells exhibited high proliferative capacity and differentiated toward cells of mesenchymal lineages with high efficiency. Bursa-derived cells expressed markers of tenocytes when treated with bone morphogenetic protein-12 (BMP-12) and assumed aligned morphology in culture. Bursa cells pretreated with BMP-12 and seeded in ceramic scaffolds formed extensive bone, as well as tendon-like tissue in vivo. Bone formation was demonstrated by histological analysis and immunofluorescence for DMP-1 in tissue sections made from the scaffolds seeded with the cells. Tendon-like tissue formed in vivo consisted of parallel collagen fibres typical of tendon tissues. Bursa-derived cells also formed a fibrocartilagenous tissue in the ceramic scaffolds. Taken together, the results demonstrate a new source of MSCs with a high potential for application in tendon repair.

Dataset Information

Adipose tissue: A natural resource for multipotent mesenchymal stem cells with potential translation to trigerminal layers.

Publications

Adipose tissue: A natural resource for multipotent mesenchymal stem cells with potential translation to trigerminal layers.

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