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Who Needs Neighbors? PKS8 Is a Stand-Alone Gene in Fusarium graminearum Responsible for Production of Gibepyrones and Prolipyrone B.

ABSTRACT: Genome sequencing of the genus Fusarium has revealed a great capacity for discovery of new natural products of potential economical and therapeutic importance. Several of these are unknown. In this study, we investigated the product of the PKS8 gene in Fusarium graminearum, which was recently linked to gibepyrones in F. fujikuroi. Genomic analyses showed that PKS8 constitutes a stand-alone gene in F. graminearum and related species. Overexpression of PKS8 resulted in production of gibepyrones A, B, D, G and prolipyrone B, which could not be detected in the wild type strain. Our results suggest that PKS8 produces the entry compound gibepyrone A, which is subsequently oxidized by one or several non-clustering cytochrome P450 monooxygenases ending with prolipyrone B.

SUBMITTER: Westphal KR 

PROVIDER: S-EPMC6225250 | biostudies-other | 2018 Sep

REPOSITORIES: biostudies-other

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Who Needs Neighbors? <i>PKS8</i> Is a Stand-Alone Gene in <i>Fusarium graminearum</i> Responsible for Production of Gibepyrones and Prolipyrone B.

Westphal Klaus Ringsborg KR   Muurmann Asmus Toftkær AT   Paulsen Iben Engell IE   Nørgaard Kim Tanja Hejselbak KTH   Overgaard Marie Lund ML   Dall Sebastian Mølvang SM   Aalborg Trine T   Wimmer Reinhard R   Sørensen Jens Laurids JL   Sondergaard Teis Esben TE  

Molecules (Basel, Switzerland) 20180902 9

Genome sequencing of the genus <i>Fusarium</i> has revealed a great capacity for discovery of new natural products of potential economical and therapeutic importance. Several of these are unknown. In this study, we investigated the product of the <i>PKS8</i> gene in <i>Fusarium graminearum</i>, which was recently linked to gibepyrones in <i>F. fujikuroi</i>. Genomic analyses showed that <i>PKS8</i> constitutes a stand-alone gene in <i>F. graminearum</i> and related species. Overexpression of <i>  ...[more]

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