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Blood Clearance of Citric Acid-Coated Superparamagnetic Iron Oxide Nanoparticles in Rats - a Pilot Study.

ABSTRACT: Superparamagnetic iron oxide nanoparticles are primarily utilized for different biomedical applications such as magnetic resonance imaging (MRI), hyperthermia, cancer treatment, targeted delivery of drugs or genes and biosensors. Nanoparticles are interesting due to their unique proprieties together with minor side effects. It is essential to determine the blood clearance of superparamagnetic nanoparticles (SPIONs) for in vivo biomedical applications, to ensure their optimum clinical use. The purpose of this study was to evaluate the elimination kinetic of citric-acid iron oxide nanoparticles in blood via intravenous injection in rats. Animals were blood sampled at different time intervals, ranging from 30 minutes to 24 hours after injection. The decay of SPIONs in blood was analyzed using electron paramagnetic resonance (EPR) technique. The results suggest that the injected iron oxide nanoparticles are rapidly cleared from circulation, with half-life of elimination process from the bloodstream about 14.06 minutes.

SUBMITTER: Trincu NF

PROVIDER: S-EPMC6243511 | biostudies-other | 2015 Oct-Dec

REPOSITORIES: biostudies-other

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Blood Clearance of Citric Acid-Coated Superparamagnetic Iron Oxide Nanoparticles in Rats - a Pilot Study.

Trincu N F NF Balșeanu T A TA Ungureanu B S BS Fifere A A Pirici I I Săftoiu A A Neamțu J J

Current health sciences journal 20151001 4

Superparamagnetic iron oxide nanoparticles are primarily utilized for different biomedical applications such as magnetic resonance imaging (MRI), hyperthermia, cancer treatment, targeted delivery of drugs or genes and biosensors. Nanoparticles are interesting due to their unique proprieties together with minor side effects. It is essential to determine the blood clearance of superparamagnetic nanoparticles (SPIONs) for in vivo biomedical applications, to ensure their optimum clinical use. The pu ...[more]

PMID: 30538834

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Project description:Superparamagnetic iron oxide nanoparticles (SPIONs) have been widely utilized for the diagnosis and therapy of specific diseases, as magnetic resonance imaging (MRI) contrast agents and drug-delivery carriers, due to their easy transportation to targeted areas by an external magnetic field. For such biomedical applications, SPIONs must have multifunctional characteristics, including optimized size and modified surface. However, the biofunctionality and biocompatibility of SPIONs with various surface functional groups of different sizes have yet to be elucidated clearly. Therefore, it is important to carefully monitor the cytotoxicity and genotoxicity of SPIONs that are surfaced-modified with various functional groups of different sizes. In this study, we evaluated SPIONs with diameters of approximately 10 nm and 100~150 nm, containing different surface functional groups. SPIONs were covered with -O? groups, so-called bare SPIONs. Following this, they were modified with three different functional groups--hydroxyl (-OH), carboxylic (-COOH), and amine (-NH?) groups--by coating their surfaces with tetraethyl orthosilicate (TEOS), (3-aminopropyl)trimethoxysilane (APTMS), TEOS-APTMS, or citrate, which imparted different surface charges and sizes to the particles. The effects of SPIONs coated with these functional groups on mitochondrial activity, intracellular accumulation of reactive oxygen species, membrane integrity, and DNA stability in L-929 fibroblasts were determined by water-soluble tetrazolium, 2',7'-dichlorodihydrofluorescein, lactate dehydrogenase, and comet assays, respectively. Our toxicological observations suggest that the functional groups and sizes of SPIONs are critical determinants of cellular responses, degrees of cytotoxicity and genotoxicity, and potential mechanisms of toxicity. Nanoparticles with various surface modifications and of different sizes induced slight, but possibly meaningful, changes in cell cytotoxicity and genotoxicity, which would be significantly valuable in further studies of bioconjugation and cell interaction for drug delivery, cell culture, and cancer-targeting applications.

Dataset Information

Blood Clearance of Citric Acid-Coated Superparamagnetic Iron Oxide Nanoparticles in Rats - a Pilot Study.

Publications

Blood Clearance of Citric Acid-Coated Superparamagnetic Iron Oxide Nanoparticles in Rats - a Pilot Study.

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